Paris/October 21
From 2007.igem.org
< Paris
Contents |
Miniprep
The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.
Transformation of Ligation products
Purification of PCR products
Digestion Reactions
| Digestion Products | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Number | Product Name | Matrix Name | Enzyme 1 | Enzyme 2 | Size | Description | |||
| D82 | frt1-CmR-frt2 FI | P16 | EcoRI | SpeI | frt1-CmR-frt2 PCR product digested for use as a FI | ||||
| D83 | frt1-CmR-frt2 BI | P16 | XbaI | PstI | frt1-CmR-frt2 PCR product digested for use as a BI | ||||
| D84 | frt1-CmR-frt2 BBi | P16 | EcoRI | PstI | frt1-CmR-frt2 PCR product digested for cloning as a BB | ||||
| D96 | frt1-CmR-frt2-T1T2 FI | P16 | EcoRI | SpeI | frt1-CmR-frt2-T1T2 PCR product digested for use as a FI | ||||
| D97 | frt1-CmR-frt2-T1T2 BI | P16 | XbaI | PstI | frt1-CmR-frt2-T1T2 PCR product digested for use as a BI | ||||
| D98 | frt1-CmR-frt2-T1T2 BBi | P16 | EcoRI | PstI | frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB | ||||
| D95 | PacI[pY-2C-IntC]SmaI | pY-2C-IntC plasmid | PacI | SmaI | pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning | ||||
