Freiburg07/primers

From 2007.igem.org

(Difference between revisions)
(Primers for calmodulin-construct with different linkerlengths)
(Primers for specific ends of the dhfr1-fragment)
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== Primers for specific ends of the dhfr1-fragment ==
== Primers for specific ends of the dhfr1-fragment ==
-
pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'
+
pr-Kpn1-dhfr1:       5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'
-
pf-Sph-h-dhfr1:5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'
+
pf-Sph-h-dhfr1:     5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'
== Primers for calmodulin-construct with different linkerlengths ==
== Primers for calmodulin-construct with different linkerlengths ==
pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'
pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'

Revision as of 13:58, 22 October 2007

Primers for specific ends of the dhfr1-fragment

pr-Kpn1-dhfr1: 5' TAATTAGGTA CCCAATTCCG GTTGTTC 3'

pf-Sph-h-dhfr1: 5' TATCGACGCA TGCACCATCA CCATCACCAT ATGGTTCGAC CATTGAAC 3'

Primers for calmodulin-construct with different linkerlengths

pf-calmo-KpnI-linker2: 5' TATCGACGGT ACCGGCGGTG AGCAGATTGC AGAGTTCAAA G 3'