Tokyo/alternative

From 2007.igem.org

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__NOTOC__
__NOTOC__
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==[[Tokyo_Tech|Abstruct]]　　[[Tokyo/Model|Concept & Model]]　　[[Tokyo/Requirements |Requirements]]　　[[Tokyo/Genetic circuit|Genetic_circuit]]　　[[Tokyo/Works|Works]]　　[[Tokyo/about our team|About_our_team]]==
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<h1> ''' Abstruct''' </h1>
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{| cellspacing="5"
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<h1> ''' 1. Abstruct''' </h1>
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A bacterial society designed in this project follows Pareto's principle as an [[Tokyo/Concept|Ant society]] does.
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To realize this model, the behaviors of the model '''- bistability of each individual and the system's stability under the coexsistence of the two types - '''have been confirmed ''in silico'' and ''in virto''. [[Tokyo/Model|See more.]]
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<br>[[Image:Tokyo_simulation_s2.jpg|500px]]
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<br>[[Image:our goal.JPG|500px]]
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This model is based on two principles - bistability and coexistence by cell-cell communication. [[Tokyo/Requirements |See more.]]
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==1.[[Tokyo/Model|Concept & Model]]==
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'''We made a bacterial society that follows Pareto's principle as an [[Tokyo/Concepts|ant society]] does. ([[Tokyo/Model|See more.]])''' [[Image:TOP.JPG|470px]]
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'''1. Coexistence　⇒　2. Worker Removal　⇒　3.Coexistence again'''
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<!--<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]-->
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==2.[[Tokyo/Requirements |Requirements]]==
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'''Bistability and coexistence by cell-cell communication are necessary to realize our model. ([[Tokyo/Requirements|See more.]])'''
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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
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==3.[[Tokyo/Genetic circuit|Genetic circuit]]==
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'''The genetic circuit in this project shown below have been consisted of [[Tokyo/new parts |our newly deviced parts]] as well as the delivered BioBrick parts. (See more for [[Tokyo/Genetic circuit|the gentic circuit]] and [[Tokyo/construction|the new parts.]])'''
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<h1>'''2. Works'''  ~ Wet & Dry ~</h1>
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<br>[[Image:Tokyo_genetic circuit_s.jpg|300px]]
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<!--<h1> ''' [[Tokyo/Experiments |Experiments]]''' </h1>-->
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[[Image:Hybridgraph.JPG|150px|]]
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===<span style="color:#ff33cc;">Wet 1</span>:　[[Tokyo/Hybrid promoter|Hybrid promoter]]===
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{| cellspacing="5"
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[[Tokyo/Hybrid promoter|The newly deviced hybrid promoter regulated by two inputs.]]- the presence of AHL and the absence of LacI, that is, the presence of IPTG.
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<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
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<br>図　概念図、配列とグラフ図
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[[Image:Hybridgraph.JPG|250px|]]
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===<span style="color:#0066ff;">Dry 1</span>: Formulation===
 
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<!--リプレッサーＡ，Ｂ，シグナル分子Ｓの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった
 
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<br>図　3つの式
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なんとかなんとか。[[Tokyo/Formulation |See more.]]
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===<span style="color:#ff33cc;">Wet 2</span>: Assay set 1===
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==4.[[Tokyo/Works|Works]]==
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'''Our model has been sophisticated though combinational wet and dry approaches; that is, by gene construction and assay accompanied by analysis and simulation. ([[Tokyo/Works|See more.]])'''
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For the parameters of Hill coefficients by AHL and IPTG assays.
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====<span style="color:#ff33cc;">Wet assay </span>====
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[[Tokyo/Assay |See more.]]
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<!--新しいパーツであるハイブリッドプロモータに関して、大腸菌を用いた実験から、2つの入力それぞれに対するHill係数を求めた。
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<br>図大腸菌の絵、FLAの絵、フィッティングのグラフ
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<br>[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
<br>[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
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====<span style="color:#0066ff;">Dry simulation </span>====
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===<span style="color:#0066ff;">Dry 2</span>: Simulation set 2===
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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なんとかなんとか。[[Tokyo/Simulation |See more.]]
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<h1> '''3. [[Tokyo/About our team |About our team]] '''</h1>
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===<span style="color:#ff33cc;">Wet 3</span>: Assay set 2===
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For the other parameters. [[Tokyo/Assay |See more.]]
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===Future works===
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Continue this combinational approaches to sophisticate our model.
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[[See more.]]
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<!--
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<h1> '''3,[[Tokyo/Achievements |Achievements ]]'''</h1>
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<br>[[Tokyo/Hybrid promoter |Hybrid promoter ]]
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<br>[[Tokyo/Bistability analisis|Bistability analysis]]
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<br>[[Tokyo/Others |Others ]]
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===*Construction===
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[[Tokyo/Genetic circuit|Genetic circuits]]
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<h1> '''3. About our team'''</h1>
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<!--[[Image:team photo.jpg]] -->
<!--[[Image:team photo.jpg]] -->
'''Students'''
'''Students'''
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<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
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'''Links'''
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[[Tokyo/sunaba |sunaba ]]
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Revision as of 13:05, 23 October 2007

Title1.JPG

Abstruct　　Concept & Model　　Requirements　　Genetic_circuit　　Works　　About_our_team

Abstruct

1.Concept & Model

We made a bacterial society that follows Pareto's principle as an ant society does. (See more.) TOP.JPG 1. Coexistence　⇒　2. Worker Removal　⇒　3.Coexistence again

2.Requirements

Bistability and coexistence by cell-cell communication are necessary to realize our model. (See more.)
Bistability.JPGCoexistence.JPG

3.Genetic circuit

The genetic circuit in this project shown below have been consisted of our newly deviced parts as well as the delivered BioBrick parts. (See more for the gentic circuit and the new parts.)


Tokyo genetic circuit s.jpg Hybridgraph.JPG


4.Works

Our model has been sophisticated though combinational wet and dry approaches; that is, by gene construction and assay accompanied by analysis and simulation. (See more.)

Wet assay


Hill AHL.JPG|Hill IPTG.JPG

Dry simulation


Tokyo simulation s2.jpg

3. About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]


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