Tokyo/Works/Assay
From 2007.igem.org
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== [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | == [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | ||
| - | + | ===Purpose: === | |
| + | <br>check if AHL activates lux-lac hybrid promoter | ||
| + | <br>check if lacI represses lux-lac hybrid promoter | ||
| + | |||
結果 | 結果 | ||
| + | |||
== [[Tokyo/IPTG assay|IPTG assay]] == | == [[Tokyo/IPTG assay|IPTG assay]] == | ||
目的 | 目的 | ||
Revision as of 16:42, 23 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
1.Important assay
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Assays for hybrid promoter
These assays were done
-to determine the characteristics of our hybrid promoter.
The data from here were used for computational analyses and simulations.
Repression and activation check with LacI and AHL
Purpose:
check if AHL activates lux-lac hybrid promoter
check if lacI represses lux-lac hybrid promoter
結果
IPTG assay
目的
結果
Activation check by cell-produced AHL
目的
結果
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
See more details
