Tokyo/Works/Assay
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<br>[[Tokyo/Works|Works top]] 0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1.[[Tokyo/Works/Formulation |Formulation]] 2.[[Tokyo/Works/Assay |Assay1]] 3.[[Tokyo/Works/Simulation |Simulation]] 4.[[Tokyo/Works/Assay2 |Assay2]] 5.[[Tokyo/Works/Future works |Future works]] | <br>[[Tokyo/Works|Works top]] 0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]] 1.[[Tokyo/Works/Formulation |Formulation]] 2.[[Tokyo/Works/Assay |Assay1]] 3.[[Tokyo/Works/Simulation |Simulation]] 4.[[Tokyo/Works/Assay2 |Assay2]] 5.[[Tokyo/Works/Future works |Future works]] | ||
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==[[Tokyo/Hill function fitting |Hill function fitting ]] == | ==[[Tokyo/Hill function fitting |Hill function fitting ]] == | ||
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導出に関してはfor more detail. | 導出に関してはfor more detail. | ||
| - | == Assays for hybrid promoter == | + | <!--== Assays for hybrid promoter == |
These assays were done | These assays were done | ||
<br>-to determine the characteristics of our hybrid promoter. | <br>-to determine the characteristics of our hybrid promoter. | ||
<br>The data from here were used for computational analyses and simulations. | <br>The data from here were used for computational analyses and simulations. | ||
| + | --> | ||
| - | == [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] == | + | === [[Tokyo/Repression and activation check with LacI and AHL|Repression and activation check with LacI and AHL]] === |
====Purpose==== | ====Purpose==== | ||
<br>check if AHL activates lux-lac hybrid promoter | <br>check if AHL activates lux-lac hybrid promoter | ||
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<br>Lux-lac hybrid promoter is activated only in the presence of AHL and IPTG. | <br>Lux-lac hybrid promoter is activated only in the presence of AHL and IPTG. | ||
| - | == [[Tokyo/IPTG assay|IPTG assay]] == | + | === [[Tokyo/IPTG assay|IPTG assay]] === |
====Purpose==== | ====Purpose==== | ||
<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | <br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | ||
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| - | =2.Preliminary assays= | + | ==2.Preliminary assays== |
'''We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.''' | '''We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.''' | ||
<br>[[Tokyo/Preliminary assays |See more details]] | <br>[[Tokyo/Preliminary assays |See more details]] | ||
Revision as of 02:55, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Hill function fitting
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
導出に関してはfor more detail.
Repression and activation check with LacI and AHL
Purpose
check if AHL activates lux-lac hybrid promoter
check if lacI represses lux-lac hybrid promoter
Result & Conclusion
AND gate by AHL & IPTG
Lux-lac hybrid promoter is activated only in the presence of AHL and IPTG.
IPTG assay
Purpose
To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells.
The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.
Result & Conclusion
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
See more details
