Tokyo/Works/Assay
From 2007.igem.org
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== [[Tokyo/IPTG assay|2.IPTG assay]] == | == [[Tokyo/IPTG assay|2.IPTG assay]] == | ||
====Purpose==== | ====Purpose==== | ||
| - | <br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. | + | <br>check if AHL activates lux-lac hybrid promoter |
| - | <br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration. | + | <!--<br>To determine the order of the concentration of IPTG necessary for the activation of our lux-lac hybrid promoter in the LacI producing pTrc99A cells. |
| + | <br> The order of the concentration is used for more detailed assay with narrower range of the IPTG concentration.--> | ||
====Result & Conclusion==== | ====Result & Conclusion==== | ||
<br>[[Image:hill IPTG.JPG|190px]] | <br>[[Image:hill IPTG.JPG|190px]] | ||
Revision as of 04:47, 24 October 2007
Works top 0.Hybrid promoter 1.Formulation 2.Assay1 3.Simulation 4.Assay2 5.Future works
Purpose of this assay (Hill function fitting)
新しく作ったハイブリッドプロモーターに関してはデータがないので，実験によりパラメータを求める．
ヒル関数をfittingさせることにより，n2,n3,K2,K3が求まる.
We have obtained data for our newly devised hybrid promoter. By Hill function fitting, we have determined n2, n3, K2, and K3.
1.AHL assay
Purpose
check if AHL activates lux-lac hybrid promoter
Result & Conclusion
Lux-lac hybrid promoter is activated in the presence of AHL.
2.IPTG assay
Purpose
check if AHL activates lux-lac hybrid promoter
Result & Conclusion
2.Preliminary assays
We conducted preliminary assays to practice experiments and check if the materials and methods worked properly.
