Tokyo/Experimental precision and accurecy
From 2007.igem.org
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===Samples: === | ===Samples: === | ||
| - | + | placQI + GFP in pTrc99A (pos. con.) | |
<br>A4 ΔP+ GFP (neg. con.) | <br>A4 ΔP+ GFP (neg. con.) | ||
Revision as of 11:18, 24 October 2007
Contents |
AHL assay
Purpose1:
To see how precisely we determine the fluorescence from GFP by removing LB media.
Samples:
placQI + GFP in pTrc99A (pos. con.)
A4 ΔP+ GFP (neg. con.)
Procedure:
AHL assay Standard protocol
Make 200 ul of mixtures of pos. con. and neg. con.
The mixture ratios of pos. con. and neg. con. are
(100:0), (70:30), (50:50), (30:70), (10:90), (3:97), (1:99)
Wash
