Tokyo/alternative

From 2007.igem.org

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[[Image:Title2.JPG|935px]]
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[[Image:Tokyotech4.JPG|900px]]
__NOTOC__
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{| cellspacing="5"
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==[[Tokyo_Tech|Abstract]]　　[[Tokyo/Model|Concept & Model]]　　[[Tokyo/Requirements |Requirements]]　　[[Tokyo/Genetic circuit|Genetic_circuit]]　　[[Tokyo/Works|Works]]　　[[Tokyo/about our team|About_our_team]]==
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<h1> ''' Abstract''' </h1>
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<h1> ''' 1. [[Tokyo/Project |Abstruct]]''' </h1>
 
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===[[Tokyo/Model|Concept & Model]]===
 
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A bacterial society designed in this project follows Pareto's principle as an [[Tokyo/Concept|Ant society]] does.
 
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To realize this model, the behaviors of the model '''- bistability of each individual and the system's stability under the coexsistence of the two types - '''have been confirmed ''in silico'' and ''in virto''.
 
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
 
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<br>[[Image:our goal.JPG|350px]]
 
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This model is based on two principles - bistability and coexistence by cell-cell communication. [[Tokyo/Requirements |See more.]]
 
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==1.[[Tokyo/Model|Concept & Model]]==
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'''We made a bacterial society that follows Pareto's principle as an [[Tokyo/Concepts|ant society]] does. ([[Tokyo/Model|See more.]])''' [[Image:TOP.JPG|470px]]
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'''1. Coexistence　⇒　2. Worker Removal　⇒　3.Coexistence again'''
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==2.[[Tokyo/Requirements |Requirements]]==
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'''Bistability and coexistence by cell-cell communication are necessary to realize our model. ([[Tokyo/Requirements|See more.]])'''
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<br>[[Image:Bistability.JPG|200px]][[Image:Coexistence.JPG|250px]]
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<h1>'''2. [[Tokyo/Works |Works]]'''</h1>
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==3.[[Tokyo/Genetic circuit|Genetic circuit]]==
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<!--<h1> ''' [[Tokyo/Experiments |Experiments]]''' </h1>-->
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===2-a. [[Tokyo/Genetic circuit|Genetic circuits]] ===
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'''The genetic circuit in this project shown below have been consisted of [[Tokyo/new parts |our newly devised parts]] as well as the delivered BioBrick parts. (See more for [[Tokyo/Genetic circuit|the gentic circuit]] and [[Tokyo/construction|the new parts.]])'''
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===2-b. Combinatione of Wet & Dry===
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<br>[[Image:Tokyo_genetic circuit_s.jpg|300px]]
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[[Image:Hybridgraph.JPG|150px|]]
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===0. <span style="color:#ff33cc;">Wet </span>:　[[Tokyo/Hybrid promoter|Hybrid promoter]]===
 
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[[Tokyo/Hybrid promoter|The newly deviced hybrid promoter regulated by two inputs.]]
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<!--New part development:Hybrid promoterPBとして、2つの入力を受けるプロモータを開発する必要があった
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<br>図　概念図、配列とグラフ図
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[[Image:Hybridgraph.JPG|200px|]]
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===1. <span style="color:#0066ff;">Dry </span>:　 [[Tokyo/Formulation |Formulation]]===
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==4.[[Tokyo/Works|Works]]==
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<!--リプレッサーＡ，Ｂ，シグナル分子Ｓの時間変化を式として表し、安定性解析を行った結果、Hill係数が大切であることがわかった
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'''Our model has been sophisticated though combinational wet and dry approaches. ([[Tokyo/Works|See more.]])'''
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<br>図　3つの式
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===2. <span style="color:#ff33cc;">Wet </span>: 　[[Tokyo/Assay |Assay set 1]]===
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====<span style="color:#ff33cc;">Wet assay </span>====
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<br>[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
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For the parameters of Hill coefficients by AHL and IPTG assays
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====<span style="color:#0066ff;">Dry simulation </span>====
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<!--新しいパーツであるハイブリッドプロモータに関して、大腸菌を用いた実験から、2つの入力それぞれに対するHill係数を求めた。
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<br>[[Image:Tokyo_simulation_s2.jpg|350px]]
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<br>図大腸菌の絵、FLAの絵、フィッティングのグラフ
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[[Image:hill AHL.JPG|190px]]|[[Image:hill IPTG.JPG|190px]]
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===3. <span style="color:#0066ff;">Dry </span>: 　[[Tokyo/Simulation |Simulation]]===
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===4. <span style="color:#ff33cc;">Wet </span>: 　[[Tokyo/Assay |Assay set 2]]===
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We tested whether our plasmids gave the other parameters to satisfy the simulation by the experiments using ''E. coli''.
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===5. Future works===
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Continue this combinational approaches to sophisticate our model.
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[[See more.]]
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<h1> '''3,[[Tokyo/Achievements |Achievements ]]'''</h1>
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<br>[[Tokyo/Hybrid promoter |Hybrid promoter ]]
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== 5. [[Tokyo/About our team |About our team]] ==
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<br>[[Tokyo/Bistability analisis|Bistability analysis]]
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<br>[[Tokyo/Others |Others ]]
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<h1> '''3. About our team'''</h1>
 
<!--[[Image:team photo.jpg]] -->
<!--[[Image:team photo.jpg]] -->
'''Students'''
'''Students'''
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<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
<br>[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]
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'''Links'''
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[[Tokyo/sunaba |sunaba ]]
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Latest revision as of 11:26, 24 October 2007

Tokyotech4.JPG

Abstract　　Concept & Model　　Requirements　　Genetic_circuit　　Works　　About_our_team

Abstract

1.Concept & Model

We made a bacterial society that follows Pareto's principle as an ant society does. (See more.) TOP.JPG 1. Coexistence　⇒　2. Worker Removal　⇒　3.Coexistence again

2.Requirements

Bistability and coexistence by cell-cell communication are necessary to realize our model. (See more.)
Bistability.JPGCoexistence.JPG

3.Genetic circuit

The genetic circuit in this project shown below have been consisted of our newly devised parts as well as the delivered BioBrick parts. (See more for the gentic circuit and the new parts.)


Tokyo genetic circuit s.jpg Hybridgraph.JPG


4.Works

Our model has been sophisticated though combinational wet and dry approaches. (See more.)

Wet assay


Hill AHL.JPG|Hill IPTG.JPG

Dry simulation


Tokyo simulation s2.jpg

5. About our team

Students
Satoru Akama | Kenichiro Iwasaki | Hiroki Kawahara | Hajime Nakatani

Advisors: Last year's member
Shotaro Ayukawa | Akio Kobayashi

Instructors
[http://www.sb.dis.titech.ac.jp/ Daisuke Kiga] | [http://www.es.dis.titech.ac.jp/ Masayuki Yamamura] | [http://nicosia.is.s.u-tokyo.ac.jp/~hagiya/ Masami Hagiya] | [http://www.sb.dis.titech.ac.jp/ Masahiko Uchiyama]


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