Tokyo/Genetic circuit

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Revision as of 12:03, 24 October 2007

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Genetic Circuit

Fig.1 is an outline of the genetic circuit in our model, designed to realize our Concept & Model.

All cells, wokers and idlers, have THE SAME genetic circuit (just as 'society' members have practically the same genetic backgrounds).

Fig.1: Genetic Circuit

Workers (State-A)

(Fig2) In the A-state cell, promoter Pa is "on", and the cell expresses repressor Ra, luxI, and RFP. Repressor Ra put the promoter Pb into "off", and prohibits to change the state. This cell also expresses luxI, which makes AHL (cell-cell communication molecule). And in the downstream of these genes, there is an RFP gene. Therefore we can detect state A cell with FACS.

Fig.2: Workers (State-A)

Idlers (State-B)

(Fig3) In the B-state cell, with the exsistence of AHL, promoter Pb is "on". And repressor Rb and GFP are expressed. Repressor Rb inhibits the expression of promoter Pa, and GFP is used to detect state-B cells.

If Workers are removed...

(Fig 4)If A-state cells are removed, B-state cells recognizes the absense of A-cells. That is, they respond to the decrease of AHL, not to produce repressor Rb. In this condition, repressions of Pa by Rb, and Pb by Ra are declined. This puts the toggle-like circuit into "reset".
(鮎川)toggle-likeという言葉はうちのgenetic circuitには当てはまらないと思います。そもそもtoggle switchにAでもBでもないresetという状態はないのでは？

Fig.3: Idlers (State-B)

Fig.4: If Workers are removed...

Hybrid promoter

これを実装するためにPbはハイブリッドプロモータである必要がある。 ⇒ハイブリッドプロモーターとは？WorkのページへHybrid promoter

他の遺伝子回路の案

コンセプトモデルを遺伝子回路にする上で、他にもいくつか方法が考えられた。 その案を以下に示す。