Freiburg07/Dephosphorylation
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Add one more 1 µl SAP.<BR> | Add one more 1 µl SAP.<BR> | ||
| - | Incubate for 20 minutes at 70°C (heat shock)<br> | + | Incubate for 20 minutes at 70°C (heat shock).<br> |
[https://2007.igem.org/Freiburg BACK] | [https://2007.igem.org/Freiburg BACK] | ||
Revision as of 15:19, 24 October 2007
Dephosphorylation
total volume: 50 µl
43 µl plasmid
1 µl SAP (shrink alkaline phosphatase)
5 µl SAP buffer (1:10)
Incubate for 30 minutes at 37°C.
Add one more 1 µl SAP.
Incubate for 20 minutes at 70°C (heat shock).
