Freiburg07/Dephosphorylation
From 2007.igem.org
(Difference between revisions)
Luckytaschka (Talk | contribs) |
Luckytaschka (Talk | contribs) |
||
| (One intermediate revision not shown) | |||
| Line 2: | Line 2: | ||
total volume: 50 µl<BR> | total volume: 50 µl<BR> | ||
| + | |||
43 µl plasmid<BR> | 43 µl plasmid<BR> | ||
1 µl SAP (shrink alkaline phosphatase)<BR> | 1 µl SAP (shrink alkaline phosphatase)<BR> | ||
| Line 10: | Line 11: | ||
Add one more 1 µl SAP.<BR> | Add one more 1 µl SAP.<BR> | ||
| - | Incubate for 20 minutes at 70°C (heat shock)<br> | + | Incubate for 20 minutes at 70°C (heat shock).<br> |
[https://2007.igem.org/Freiburg BACK] | [https://2007.igem.org/Freiburg BACK] | ||
Latest revision as of 15:20, 24 October 2007
Dephosphorylation
total volume: 50 µl
43 µl plasmid
1 µl SAP (shrink alkaline phosphatase)
5 µl SAP buffer (1:10)
Incubate for 30 minutes at 37°C.
Add one more 1 µl SAP.
Incubate for 20 minutes at 70°C (heat shock).
