Freiburg07/labnotes2
From 2007.igem.org
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| + | == Wed, 25th July to Thu, 03th August 2007 == | ||
| + | we cloned the ''320d blac1-wz-dhfr'' plasmid once again and repeated the PCR for the dhfr1 peace to create the plasmid again | ||
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| + | == Wed, 10th July to Fri, 20th July 2007 == | ||
| + | After ligating the dhfr1 fragment into the dhfr2 including vector and making several digests we came to the conclusion that something was wrong with our plasmids. sequencing the construct affirmed this<br> | ||
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== Mon, 25th June to Thu, 05th July 2007 == | == Mon, 25th June to Thu, 05th July 2007 == | ||
we gained the dhfr1 fragment by using PCR. we used an inproper plasmid for the first PCR so we had to repeat it several times, using another plasmid<br> | we gained the dhfr1 fragment by using PCR. we used an inproper plasmid for the first PCR so we had to repeat it several times, using another plasmid<br> | ||
Revision as of 21:29, 24 October 2007
Contents |
Wed, 25th July to Thu, 03th August 2007
we cloned the 320d blac1-wz-dhfr plasmid once again and repeated the PCR for the dhfr1 peace to create the plasmid again
Wed, 10th July to Fri, 20th July 2007
After ligating the dhfr1 fragment into the dhfr2 including vector and making several digests we came to the conclusion that something was wrong with our plasmids. sequencing the construct affirmed this
Mon, 25th June to Thu, 05th July 2007
we gained the dhfr1 fragment by using PCR. we used an inproper plasmid for the first PCR so we had to repeat it several times, using another plasmid
Wed, 20th June to Thu, 28th June 2007
Planning a new Plasmid containing splitted Dhfr Fragments instead of the lactamase
we cutted the 320d blac1-wz-blac2 plasmid (used as vector) and a 300d dhfr2 plasmid (insert) with HindIII and SpeI, ligated them and transformed into XL-1 blue E.coli cells to get a 320d blac1-wz-dhfr2 plasmid
