Tokyo/Works/Assay0
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===Samples: === | ===Samples: === | ||
| - | <br>1. pTrc99A + [ | + | <br>1. pTrc99A + [Lux lac hybrid promoter – GFP] (LacI+) |
| - | <br>2. pBR322 TetR + [ | + | <br>2. pBR322 TetR + [Lux lac hybrid promoter – GFP] (lacI-) |
| - | <br>3. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) | + | <br>3. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.) |
| - | <br>4. pTrc99A + [LacI promoter – GFP] | + | <br>4. pTrc99A + [LacI promoter – GFP] (Neg. con.) |
| - | <br>5. pBR322 TetR + [LacI promoter – GFP] | + | <br>5. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check) |
<br>'''Repressor LacI expression:''' | <br>'''Repressor LacI expression:''' | ||
Revision as of 04:20, 25 October 2007
Works top 0. Hybrid promoter 1. Formulation 2. Assay1 3. Simulation 4. Assay2 5. Future works
Purpose 1:
To check if LacI hybrid promoter is activated by AHL and repressed by LacI.
Purpose 2:
To obtain parameters of LacI hybrid promoter for computational simulation.
Samples:
1. pTrc99A + [Lux lac hybrid promoter – GFP] (LacI+)
2. pBR322 TetR + [Lux lac hybrid promoter – GFP] (lacI-)
3. pTrc99A + [placQI – GFP] (placQI is constitutive promoter) (Pos. con.)
4. pTrc99A + [LacI promoter – GFP] (Neg. con.)
5. pBR322 TetR + [LacI promoter – GFP] (Neg. con. check)
Repressor LacI expression:
pTrc99A expresses LacI
pBR322 TetR does NOT express LacI
Antibiotics resistance:
pTrc99A gives ampicillin-resistance
pBR322 TetR gives ampicillin-resistance
[LacI hybrid promoter – GFP] gives kanamicin-resistance
[placQI – GFP] gives kanamicin-resistance
Procedure:
Results and Conclusion
