USTC

From 2007.igem.org

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<font color="#bb0000">Cosumption</font>
<font color="#bb0000">Cosumption</font>
 +
'''4mL LB medium consumption'''<br>
'''4mL LB medium consumption'''<br>
-
> 1400 tubes
+
> 1400 tubes<br>
'''Competence Strain we've made'''<br>
'''Competence Strain we've made'''<br>
-
> 300 EP tubes
+
> 300 EP tubes<br>
'''96-Well Plates we've used'''<br>
'''96-Well Plates we've used'''<br>
> 10 plates
> 10 plates
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<font color="#bb0000">Detection</font>
<font color="#bb0000">Detection</font>
'''Colony-PCR detection'''<br>
'''Colony-PCR detection'''<br>
-
> 500 times
+
> 500 times<br>
'''Fluorescence detection'''<br>
'''Fluorescence detection'''<br>
-
> 100 times
+
> 100 times<br>
'''DNA sequencing'''<br>
'''DNA sequencing'''<br>
> 130 times
> 130 times
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<font color="#bb0000">Measurement</font>
<font color="#bb0000">Measurement</font>
'''Fluorescent Density measuring'''<br>
'''Fluorescent Density measuring'''<br>
-
> 200 times
+
> 200 times<br>
'''ONPG assay'''<br>
'''ONPG assay'''<br>
-
> 70 times
+
> 70 times<br>

Revision as of 15:54, 25 October 2007


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements and favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.

A demonstration system has also been assembled to show the practicality of this method.Just like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in future?


DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project

Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Statistics

In less than 5 months, our

Cosumption

4mL LB medium consumption
> 1400 tubes
Competence Strain we've made
> 300 EP tubes
96-Well Plates we've used
> 10 plates


Detection Colony-PCR detection
> 500 times
Fluorescence detection
> 100 times
DNA sequencing
> 130 times


Measurement Fluorescent Density measuring
> 200 times
ONPG assay
> 70 times

Members

Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun



Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]

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