From 2007.igem.org
(Difference between revisions)
|
|
| Line 85: |
Line 85: |
| | | | |
| | |width=240px style="padding: 5px; background-color: #ccccff" | | | |width=240px style="padding: 5px; background-color: #ccccff" | |
| - | <!---
| |
| - | <h3>Events</h3>
| |
| | | | |
| - | '''25/10/2007'''<br>
| |
| - | Hard documenting ......
| |
| - |
| |
| - | '''18/09/2007'''<br>
| |
| - | [[USTC/JamboreeSchedule|Draft of our schedule for the Jamboree]].
| |
| - |
| |
| - | '''04/09/2007'''<br>
| |
| - | The second scheme of NAND gate has been built up and the rough data show that it works.
| |
| - | More precise quantitative data is to be measured.
| |
| - |
| |
| - | '''02/09/2007'''<br>
| |
| - | Double-reporters system version 2 has passed sequencing, and been confirmed by X-gal assay
| |
| - | and fluorescence observation.
| |
| - |
| |
| - | [[USTC/Previous|'''Previous''']]
| |
| - |
| |
| - | |width=240px style="padding: 5px; background-color: #ccff99" |
| |
| - |
| |
| - | --->
| |
| | <h3>Members</h3> | | <h3>Members</h3> |
| | | | |
Revision as of 17:15, 25 October 2007
Today = new Date();
Jamboree = new Date("November 3, 2007");
msInADay = 1000 * 60 * 60 * 24;
display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay);
document.write(" " + display +" ");
// </script></html>days left until the Jamboree!
</font> -->
Our Project:
Extensible Logic Circuit in Bacteria
Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.
Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small piece of DNA containing cis-acting elements and favored for its small scale and potential to implement complicated logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specified DNA-recognition regions are able to transmit signals without mutual interference, just as copper wires. In this way, a circuit can be constructed regardless of the number of logic gates and the manner of connections between the wires.
A demonstration system has also been assembled to show the practicality of this method.Just like Digital Electronic Circuits in early days, it is simple and ugly. But how will it appear in future?
|
From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian
|
Project
For detailed description, please click each hyperlink below.
Introduction
Core Logic Components:
Logic-Gate Promoters
Wires without Interference:
Repressor Evolution on Plates
Repressor Evolution in Silico
Peripheral Devices:
Inputs and Outputs
Extensible System:
A Demonstration
Further More
|
Members
4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu
3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun
3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou
|
Statistics
In more than 4 months, our
Parts designed
= 229 parts ...[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Go to see them]
Parts submitted
= 123 parts
Oligo-synthesis
~ 120 fragments
PCR synthesizing and detection
~ 800 times
Restriction digestion
> 600 times
Transformation
~ 300 times
DNA sequencing
~ 130 times
Fluorescence detection
> 100 times
Fluorescent Density measuring
> 200 times
ONPG assay
~ 70 times
|
Resources
Modeling Utilities
Protocols
Sponsors
Service Providers
|
Gallery
Team Photos
Laboratory
[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]
[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]
|
Links
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]
[http://partsregistry.org iGEM Standard Parts' Catalog ]
IGEM2007 Team List
[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]
|
