USTC

From 2007.igem.org

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<h3>Statistics</h3>
<h3>Statistics</h3>
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In more than 4 months, our
 
'''Parts designed'''<br>
'''Parts designed'''<br>
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-
'''Oligo-synthesis'''<br>
+
'''PCR'''<br>
-
~ 120 fragments<br>
+
~ 800 reactions<br>
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'''PCR synthesizing and detection'''<br>
+
-
~ 800 times<br>
+
'''Restriction digestion'''<br>
'''Restriction digestion'''<br>
-
> 600 times<br>
+
> 600 reactions<br>
'''Transformation'''<br>
'''Transformation'''<br>
~ 300 times
~ 300 times
-
 
'''DNA sequencing'''<br>
'''DNA sequencing'''<br>
~ 130 times<br>
~ 130 times<br>
-
'''Fluorescence detection'''<br>
+
'''Fluorescence measurement'''<br>
-
> 100 times<br>
+
> 100 assays<br>
-
'''Fluorescent Density measuring'''<br>
+
-
> 300 times<br>
+
'''ONPG assay'''<br>
'''ONPG assay'''<br>
~ 100 times<br>
~ 100 times<br>

Revision as of 19:43, 25 October 2007


USTC Logo.png

Today = new Date(); Jamboree = new Date("November 3, 2007"); msInADay = 1000 * 60 * 60 * 24; display = Math.floor((Jamboree.getTime() - Today.getTime())/msInADay); document.write(" " + display +" "); // </script></html>days left until the Jamboree! </font> -->

Our Project:
Extensible Logic Circuit in Bacteria

Artificial Bio-Logic Circuit is composed of "logic gates" and "wires" like Digital Electronic Circuits. Though we have been enjoying the advantages of ultra-large-scale electronic circuits in modern life, we still cannot implement a somewhat small-scale circuit in vivo with several levels of gates.

Our project is to provide a new method for building up a fully extensible bio-logic circuit in bacteria. A small fragment of DNA containing cis-acting elements, favored for its small scale and potential to implement complex logic computation in vivo, can be systematically built up and act as a gate. Meanwhile, artificial repressors with highly-specific DNA-recognition regions are able to transmit signals without mutual interference, just as enameled wires. In this way, a circuit can be constructed regardless of the number of logic gates and the layout of the wires.

A demonstration system has also been assembled to show the practicality of this method. Just like Digital Electronic Circuits in early days, it is simple and ugly. Nevertheless, how will it appear in future?

For detailed description, please click each hyperlink below.

DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Project


Introduction


Core Logic Components:

Logic-Gate Promoters


Wires without Interference:

Repressor Evolution on Plates

Repressor Evolution in Silico


Peripheral Devices:

Inputs and Outputs


Extensible System:

A Demonstration

Further More


Members


4 Graduates:
Zhan Jian
Ma Rui
Ding Bo
Ma Xiaoyu


3 Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun


3 Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Statistics

Parts designed
= 229 parts . . . [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Go to see them]
Parts submitted
= 123 parts


PCR
~ 800 reactions
Restriction digestion
> 600 reactions
Transformation
~ 300 times


DNA sequencing
~ 130 times
Fluorescence measurement
> 100 assays
ONPG assay
~ 100 times


Events

Resources

Modeling Utilities

Protocols

Sponsors

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]

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