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Revision as of 00:21, 8 June 2007 (view source) Samanthaliang (Talk | contribs) ← Older edit		Latest revision as of 22:50, 20 June 2007 (view source) Samanthaliang (Talk | contribs)
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	'''[rbs][Cre]''' <br>		'''[rbs][Cre]''' <br>
-	'''rbs variants Bca1090''' <br>	+	'''rbs library - want a weak rbs actually''' <br>
-	'''2 Different Cre variants (212 and 213)''' <br>	+	'''3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)''' <br>
	----		----
		+	'''ATG Cre variant''' <br>
		+
		+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
		+	Digest pBca9145-Bca1117  (EcoRI/BglII, 3101+9bp, Large)<br>
		+	Plate on CamAmp <br>
		+	Product is pBca9145-I716224. <br>
		+
		+
		+	'''GTG Cre variant''' <br>
		+
		+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
		+	Digest pBca9145-I716212  (EcoRI/BglII, 3101+9bp, Large)<br>
		+	Plate on CamAmp  <br>
		+	Product is pBca9145-I716225. <br>
-	Digest pBca9145-Bca1090 (BamHI/BglI/AlwNI, 942+596+553bp, Large) <br>
-	Digest pBca9145-I716212 (BglII/BglI, 2187+923bp, Small) <br>
-	Product is pBca9145-I716217. <br>
		+	'''TTG Cre variants''' <br>
-	Digest pBca9145-Bca1090  (BamHI/BglI, 942+596+553bp, Large) <br>	+	Digest pBca1121-Bca9230  (EcoRI/BamHI, 2927+955bp, Small) <br>
-	Digest pBca9145-I716213  (BglII/BglI, 2187+923bp, Small)<br>	+	Digest pBca9145-I716213  (EcoRI/BglII, 3101+9bp, Large)<br>
-	Product is pBca9145-I716221. <br>	+	Plate on CamAmp <br>
		+	Product is pBca9145-I716226. <br>

---

Latest revision as of 22:50, 20 June 2007

[rbs][Cre]
rbs library - want a weak rbs actually
3 Different Cre variants (ATG Bca1117, GTG I716212 and TTG I716213)

---

ATG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-Bca1117 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716224.

GTG Cre variant

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716212 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716225.

TTG Cre variants

Digest pBca1121-Bca9230 (EcoRI/BamHI, 2927+955bp, Small)
Digest pBca9145-I716213 (EcoRI/BglII, 3101+9bp, Large)
Plate on CamAmp
Product is pBca9145-I716226.