Chiba/Project Design

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Revision as of 14:28, 26 October 2007

Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿

Project Design

Concept

ものがたり
 社団法人　農林水産技術情報協会http://www.afftis.or.jp/index.html
ばらばらになっている大腸菌を一ヶ所に集めて吸着させて、まりものような球体の大腸菌の集合体を作ることを目指した。(We aimed to make a spherical gathering of bacteria such like marimo by scattered bacteria aggregating and sticking each other.)

What our system requires

1.Sticky Tag

Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds with metal ions) on the flagellar fillament.
鞭毛にヒスタグをディスプレイし，金属イオンを介して，バクテリアを接着することを目指した．

Fig1. His-tagged flagella as a bactria linker. 鞭毛は便宜上１本しか描いてないが実際は1~10本/細胞．

2.Communication

違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてsticky handsを生やす．そして合体．

Fig2. Bacterial Communication.

3.Size Control

Make an AHL concentration gradient for quorum sensing.

Fig3. AHL concentration gradient.

How Our System Works

Senders stick to each other by way of metal ions using flagella displaying histidine tags. This becomes the core of Marimo. They also produce AHL to call receiver cells.
Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).
Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.
This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO.

@@ Line 36: / Line 36: @@
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-#Senderは常にヒスタグをディスプレイした鞭毛を発現させて金属イオンを介してSender同士が接着します。またSenderは常にAHLを合成します。<br>
+#Senders stick to each other by way of metal ions using flagella displaying histidine tags. This becomes the core of Marimo. They also produce AHL to call receiver cells.<br>
-#AHLが届く範囲にいるReceiverはGFPを発現し、ヒスタグをディスプレイした鞭毛を発現します。<br>
+#Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).<br>
-#ReceiverはSenderの周りに金属イオンを介して接着します。<br>
+#Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.<br>
-#しかしこのままでは大腸菌の集合体が再現なく大きくなってしまいます。そこでReceiverはAHLを分解します。AHLの拡散が抑えられ、鞭毛が生えるReceiverの数が制限されるので大きさが有限に定まります。<br><br>
+#This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO.<br><br>
-#Senders stick to each other by way of metal ions using flagella displaying histidines. They also produce AHL.<br>
-#Receivers express his/flagella and GFP near senders (in high [AHL]).<br>
-#Receivers stick around senders by way of metal ions.<br>
-#This way, bacteria cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO.<br><br>

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