Week 16

From 2007.igem.org

(Difference between revisions)
 
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Line 1: Line 1:
::'''10/15/07'''
::'''10/15/07'''
*O/N ligations for:
*O/N ligations for:
-
::-I763019;
+
::-[http://partsregistry.org/Part:BBa_I763019 I763019];
-
::-pTet-LacI-T-Plambda-RFP.
+
::-[http://partsregistry.org/Part:BBa_I763039 I763039].
   
   
Line 8: Line 8:
::'''10/16/07'''
::'''10/16/07'''
-
*Transformation of I763019 and pTet-LacI-T-Plambda-RFP.
+
*Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039].
*We inoculate:
*We inoculate:
-
::-I763005;
+
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
-
::-I763015;
+
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
-
::-I763004.
+
::-[http://partsregistry.org/Part:BBa_I763004 I763004].
-
*Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
+
*Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
::'''10/17/07'''
::'''10/17/07'''
*Miniprep of:
*Miniprep of:
-
::-I763005;
+
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
-
::-I763015;
+
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
-
::-I763004.
+
::-[http://partsregistry.org/Part:BBa_I763004 I763004].
*We inoculate:
*We inoculate:
-
::-Ptet-lacI-T-Plambda-RFP-pLac-CI-Lacy-gfp;
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029];
-
::-pTet-LacI-T-pLambda-rfp (high copy).
+
::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
-
*Control Digestion for results impagination (X/P):
+
*Control Digestion of the following Minipreps for results impagination:
-
::- I763020;
+
::-[http://partsregistry.org/Part:BBa_I763020 I763020];
-
::- I763004;
+
::-[http://partsregistry.org/Part:BBa_I763004 I763004];
-
::- I763011;
+
::-[http://partsregistry.org/Part:BBa_I763011 I763011];;
-
::- I763007 (on high copy plasmid);
+
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid);
-
::- I763007 (on low copy plasmid);
+
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);
-
::- I763033;
+
::-[http://partsregistry.org/Part:BBa_I763033 I763033];
-
::- I763015;
+
::-[http://partsregistry.org/Part:BBa_I763015 I763015];
-
::- I763012;
+
::-[http://partsregistry.org/Part:BBa_I763012 I763012];
-
::- I763005;
+
::-[http://partsregistry.org/Part:BBa_I763005 I763005];
-
::- I763031.
+
::-[http://partsregistry.org/Part:BBa_I763031 I763031].
 +
*Gel electrophoresis of the fragments above.
Line 40: Line 41:
::'''10/18/07'''
::'''10/18/07'''
*Miniprep:
*Miniprep:
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp;  
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029];  
-
::-pTet-LacI-T-pLambda-rfp (high copy).  
+
::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).  
*Digestion for:
*Digestion for:
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
*Band extraction of:
*Band extraction of:
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
*Ligation for:
*Ligation for:
-
::-pSB4A3 (E/P)
+
::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp (E/P)
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
*Ligation for:
*Ligation for:
-
::-pLac-GFP(X/P)
+
::-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)
-
- pTetR-LacI (S/P)
+
::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)
-
*Control Digestion for results impagination:
+
*Control Digestion of the following Minipreps for results impagination:
-
::- I763016 (low copy plasmid);
+
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
-
::- I763016 (high copy plasmid);
+
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
-
::- I763036;
+
::- [http://partsregistry.org/Part:BBa_I763036 I763036];
-
::- I763019;
+
::- [http://partsregistry.org/Part:BBa_I763019 I763019];
-
::- I763032;
+
::- [http://partsregistry.org/Part:BBa_I763032 I763032];
-
::- I763025 (low copy plasmid);
+
::- [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
-
::- I763025 (high copy plasmid);
+
::- [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
-
::- I763026;
+
::- [http://partsregistry.org/Part:BBa_I763026 I763026];
-
::- I763035.  
+
::- [http://partsregistry.org/Part:BBa_I763035 I763035].  
 +
*Gel electrophoresis of the fragments above.
Line 73: Line 75:
::'''10/19/07'''
::'''10/19/07'''
*Transformation:
*Transformation:
-
::-pLac-GFP-pTet-LacI (on high copy plasmid);::
+
::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
-
::-pTet-LacI-T-Plambda-RFP;
+
::-[http://partsregistry.org/Part:BBa_I763039 I763039];
-
::-Ptet-lacI-T.Plambda.RFP-pLac-CI-Lacy-gfp on pSB4A3 (low copy plasmid).
+
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
-
[[Bologna | Back]]
+
[[Bologna#Diary | Back]]

Latest revision as of 15:50, 26 October 2007

10/15/07
  • O/N ligations for:
-[http://partsregistry.org/Part:BBa_I763019 I763019];
-[http://partsregistry.org/Part:BBa_I763039 I763039].



10/16/07
  • Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039].
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763005 I763005];
-[http://partsregistry.org/Part:BBa_I763015 I763015];
-[http://partsregistry.org/Part:BBa_I763004 I763004].
  • Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.


10/17/07
  • Miniprep of:
-[http://partsregistry.org/Part:BBa_I763005 I763005];
-[http://partsregistry.org/Part:BBa_I763015 I763015];
-[http://partsregistry.org/Part:BBa_I763004 I763004].
  • We inoculate:
-[http://partsregistry.org/Part:BBa_I763029 I763029];
-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
  • Control Digestion of the following Minipreps for results impagination:
-[http://partsregistry.org/Part:BBa_I763020 I763020];
-[http://partsregistry.org/Part:BBa_I763004 I763004];
-[http://partsregistry.org/Part:BBa_I763011 I763011];;
-[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);
-[http://partsregistry.org/Part:BBa_I763033 I763033];
-[http://partsregistry.org/Part:BBa_I763015 I763015];
-[http://partsregistry.org/Part:BBa_I763012 I763012];
-[http://partsregistry.org/Part:BBa_I763005 I763005];
-[http://partsregistry.org/Part:BBa_I763031 I763031].
  • Gel electrophoresis of the fragments above.


10/18/07
  • Miniprep:
-[http://partsregistry.org/Part:BBa_I763029 I763029];
-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
  • Digestion for:
-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
  • Band extraction of:
-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
  • Ligation for:
-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)
-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
  • Ligation for:
-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)
-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)
  • Control Digestion of the following Minipreps for results impagination:
- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
- [http://partsregistry.org/Part:BBa_I763036 I763036];
- [http://partsregistry.org/Part:BBa_I763019 I763019];
- [http://partsregistry.org/Part:BBa_I763032 I763032];
- [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
- [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
- [http://partsregistry.org/Part:BBa_I763026 I763026];
- [http://partsregistry.org/Part:BBa_I763035 I763035].
  • Gel electrophoresis of the fragments above.


10/19/07
  • Transformation:
-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
-[http://partsregistry.org/Part:BBa_I763039 I763039];
-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).



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