Week 16
From 2007.igem.org
(Difference between revisions)
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::'''10/16/07''' | ::'''10/16/07''' | ||
| - | *Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and[http://partsregistry.org/Part:BBa_I763039 I763039]. | + | *Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039]. |
*We inoculate: | *We inoculate: | ||
::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ||
::-[http://partsregistry.org/Part:BBa_I763015 I763015]; | ::-[http://partsregistry.org/Part:BBa_I763015 I763015]; | ||
::-[http://partsregistry.org/Part:BBa_I763004 I763004]. | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]. | ||
| - | *Trasformation of I763019, subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock. | + | *Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock. |
| Line 23: | Line 23: | ||
*We inoculate: | *We inoculate: | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763039 I763039](high copy). | + | ::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy). |
| - | *Control Digestion for results impagination | + | *Control Digestion of the following Minipreps for results impagination: |
::-[http://partsregistry.org/Part:BBa_I763020 I763020]; | ::-[http://partsregistry.org/Part:BBa_I763020 I763020]; | ||
::-[http://partsregistry.org/Part:BBa_I763004 I763004]; | ::-[http://partsregistry.org/Part:BBa_I763004 I763004]; | ||
::-[http://partsregistry.org/Part:BBa_I763011 I763011];; | ::-[http://partsregistry.org/Part:BBa_I763011 I763011];; | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763007 I763007](on high copy plasmid); | + | ::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid); |
::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid); | ::-[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid); | ||
::-[http://partsregistry.org/Part:BBa_I763033 I763033]; | ::-[http://partsregistry.org/Part:BBa_I763033 I763033]; | ||
| Line 35: | Line 35: | ||
::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ::-[http://partsregistry.org/Part:BBa_I763005 I763005]; | ||
::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ::-[http://partsregistry.org/Part:BBa_I763031 I763031]. | ||
| + | *Gel electrophoresis of the fragments above. | ||
| Line 41: | Line 42: | ||
*Miniprep: | *Miniprep: | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ::-[http://partsregistry.org/Part:BBa_I763029 I763029]; | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763039 I763039](high copy). | + | ::-[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy). |
*Digestion for: | *Digestion for: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] ( | + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1) |
*Band extraction of: | *Band extraction of: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] ( | + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1) |
*Ligation for: | *Ligation for: | ||
| - | ::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] ( | + | ::-[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1) |
| - | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] ( | + | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1) |
*Ligation for: | *Ligation for: | ||
| - | ::-[http://partsregistry.org/Part:BBa_I763004 I763004] ( | + | ::-[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1) |
| - | ::-[http://partsregistry.org/Part:BBa_I763026 I763026] ( | + | ::-[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1) |
| - | *Control Digestion for results impagination: | + | *Control Digestion of the following Minipreps for results impagination: |
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); | ::- [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid); | ||
::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid); | ::- [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid); | ||
| Line 68: | Line 69: | ||
::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ::- [http://partsregistry.org/Part:BBa_I763035 I763035]. | ||
| + | *Gel electrophoresis of the fragments above. | ||
| Line 73: | Line 75: | ||
::'''10/19/07''' | ::'''10/19/07''' | ||
*Transformation: | *Transformation: | ||
| - | ::-[http://partsregistry.org/Part: | + | ::-[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid); |
::-[http://partsregistry.org/Part:BBa_I763039 I763039]; | ::-[http://partsregistry.org/Part:BBa_I763039 I763039]; | ||
::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid). | ::-[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid). | ||
| Line 80: | Line 82: | ||
| - | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:50, 26 October 2007
- 10/15/07
- O/N ligations for:
- -[http://partsregistry.org/Part:BBa_I763019 I763019];
- -[http://partsregistry.org/Part:BBa_I763039 I763039].
- 10/16/07
- Transformation of [http://partsregistry.org/Part:BBa_I763019 I763019] and [http://partsregistry.org/Part:BBa_I763039 I763039].
- We inoculate:
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763004 I763004].
- Trasformation of [http://partsregistry.org/Part:BBa_I763019 I763019], subsequently growth in 2 mL of LB medium to test if fresh transformed cells have the same problem of vitality seen using the stock.
- 10/17/07
- Miniprep of:
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763004 I763004].
- We inoculate:
- -[http://partsregistry.org/Part:BBa_I763029 I763029];
- -[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
- Control Digestion of the following Minipreps for results impagination:
- -[http://partsregistry.org/Part:BBa_I763020 I763020];
- -[http://partsregistry.org/Part:BBa_I763004 I763004];
- -[http://partsregistry.org/Part:BBa_I763011 I763011];;
- -[http://partsregistry.org/Part:BBa_I763007 I763007] (on high copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763007 I763007] (on low copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763033 I763033];
- -[http://partsregistry.org/Part:BBa_I763015 I763015];
- -[http://partsregistry.org/Part:BBa_I763012 I763012];
- -[http://partsregistry.org/Part:BBa_I763005 I763005];
- -[http://partsregistry.org/Part:BBa_I763031 I763031].
- Gel electrophoresis of the fragments above.
- 10/18/07
- Miniprep:
- -[http://partsregistry.org/Part:BBa_I763029 I763029];
- -[http://partsregistry.org/Part:BBa_I763039 I763039] (high copy).
- Digestion for:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
- Band extraction of:
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
- Ligation for:
- -[http://partsregistry.org/Part:pSB4A3 pSB4A3] (Eco/Pst1)
- -[http://partsregistry.org/Part:BBa_I763029 I763029] (Eco/Pst1)
- Ligation for:
- -[http://partsregistry.org/Part:BBa_I763004 I763004] (Xba/Pst1)
- -[http://partsregistry.org/Part:BBa_I763026 I763026] (Spe/Pst1)
- Control Digestion of the following Minipreps for results impagination:
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763016 I763016] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763036 I763036];
- - [http://partsregistry.org/Part:BBa_I763019 I763019];
- - [http://partsregistry.org/Part:BBa_I763032 I763032];
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (low copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763025 I763025] (high copy plasmid);
- - [http://partsregistry.org/Part:BBa_I763026 I763026];
- - [http://partsregistry.org/Part:BBa_I763035 I763035].
- Gel electrophoresis of the fragments above.
- 10/19/07
- Transformation:
- -[http://partsregistry.org/Part:BBa_I763034 I763034] (on high copy plasmid);
- -[http://partsregistry.org/Part:BBa_I763039 I763039];
- -[http://partsregistry.org/Part:BBa_I763029 I763029] on [http://partsregistry.org/Part:pSB4A3 pSB4A3] (low copy plasmid).
