From 2007.igem.org

Revision as of 13:36, 27 August 2007 (view source) Francesca.bugane2 (Talk | contribs) ← Older edit		Latest revision as of 15:52, 26 October 2007 (view source) Silvia.tamarri2 (Talk | contribs)
(10 intermediate revisions not shown)
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	::'''08/20/07'''		::'''08/20/07'''
-	*I763010 and C0012 trasformation.	+	*[http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.
	::'''08/21/07'''		::'''08/21/07'''
-	*There isn't colonies for I763010 and C0012. we can't amplify this biobrick.	+	*There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
-	*J04631 digestion.	+	*[http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
	*Band extraction.		*Band extraction.
-	*R0051+P0412 (I763010)ligation.	+	*[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.
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	::'''08/22/07'''		::'''08/22/07'''
-	*There isn't any colony for R0051+P0412 (I763010)	+	*There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])
	::'''08/23/07'''		::'''08/23/07'''
-	*A new R0051+P0412 (I763010) ligation a new protocol (3h 15°C and 1.2 ul Ligasi).	+	*A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
-	*R0051, I763012 digestion with Spe/Pst1.	+	*[http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
-	*P0412, J04631 digestion with Xba/Pst1.	+	*[http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
	*Run and extraction from gel.		*Run and extraction from gel.
-	*Trasformation of R0051+P0412 (I763010) ligations.	+	*Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.
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	::'''08/24/07'''		::'''08/24/07'''
		+	*We plan new amplifications.
-		+	[[Bologna#Diary | Back]]
-	[[Bologna | Back]]	+

---

Latest revision as of 15:52, 26 October 2007

08/20/07

• [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.

08/21/07

• There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
• [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
• Band extraction.
• [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.

08/22/07

• There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])

08/23/07

• A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
• [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
• [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
• Run and extraction from gel.
• Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.

08/24/07

• We plan new amplifications.

Back