Week 8
From 2007.igem.org
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::'''08/20/07''' | ::'''08/20/07''' | ||
| - | *I763010 and C0012 | + | *[http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation. |
::'''08/21/07''' | ::'''08/21/07''' | ||
| - | *There | + | *There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks. |
| - | *J04631 digestion. | + | *[http://partsregistry.org/Part:BBa_J04631 J04631] digestion. |
*Band extraction. | *Band extraction. | ||
| - | *R0051+P0412 (I763010)ligation. | + | *[http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation. |
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::'''08/22/07''' | ::'''08/22/07''' | ||
| - | *There | + | *There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) |
::'''08/23/07''' | ::'''08/23/07''' | ||
| - | *A new R0051+P0412 (I763010) ligation with a new protocol (3h 15°C and 1. | + | *A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase). |
| - | *R0051, I763012 digestion with Spe/Pst1. | + | *[http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1. |
| - | *P0412, J04631 digestion with Xba/Pst1. | + | *[http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1. |
*Run and extraction from gel. | *Run and extraction from gel. | ||
| - | *Transformation of R0051+P0412 (I763010) ligations. | + | *Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations. |
| Line 33: | Line 33: | ||
| - | [[Bologna | Back]] | + | [[Bologna#Diary | Back]] |
Latest revision as of 15:52, 26 October 2007
- 08/20/07
- [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.
- 08/21/07
- There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
- [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
- Band extraction.
- [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.
- 08/22/07
- There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])
- 08/23/07
- A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
- [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
- [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
- Run and extraction from gel.
- Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.
- 08/24/07
- We plan new amplifications.
