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Latest revision as of 15:52, 26 October 2007

08/20/07

• [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012] transformation.

08/21/07

• There are no colonies for [http://partsregistry.org/Part:BBa_I763010 I763010] and [http://partsregistry.org/Part:BBa_C0012 C0012]. We can't amplify these biobricks.
• [http://partsregistry.org/Part:BBa_J04631 J04631] digestion.
• Band extraction.
• [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation.

08/22/07

• There are no colonies for [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010])

08/23/07

• A new [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligation with a new protocol (3h 15°C and 1.2ul T4 Ligase).
• [http://partsregistry.org/Part:BBa_R0051 R0051], [http://partsregistry.org/Part:BBa_I763012 I763012] digestion with Spe/Pst1.
• [http://partsregistry.org/Part:BBa_P0412 P0412], [http://partsregistry.org/Part:BBa_J04631 J04631] digestion with Xba/Pst1.
• Run and extraction from gel.
• Transformation of [http://partsregistry.org/Part:BBa_R0051 R0051]+[http://partsregistry.org/Part:BBa_P0412 P0412] ([http://partsregistry.org/Part:BBa_I763010 I763010]) ligations.

08/24/07

• We plan new amplifications.

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