Chiba/Project Design
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===How Our System Works=== | ===How Our System Works=== | ||
[[Image:Design.PNG|240x120px]][[Image:Design2.PNG|240x120px]][[Image:Design3.PNG|240x120px]][[Image:Design4.PNG|240x120px]]<br> | [[Image:Design.PNG|240x120px]][[Image:Design2.PNG|240x120px]][[Image:Design3.PNG|240x120px]][[Image:Design4.PNG|240x120px]]<br> | ||
| - | #Senders stick to each other | + | #Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Marimo. They also produce AHL to call receiver cells.<br> |
#Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).<br> | #Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).<br> | ||
#Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.<br> | #Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.<br> | ||
Revision as of 17:54, 26 October 2007
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Project Design
Concept
(photo taken from Agriculture, Forestry and Fishes Technical Information Society, Japan, http://www.afftis.or.jp/index.html)
We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.
What our system requires
1.Sticky TagMake a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds with metal ions) on the flagellar fillament.  Fig1. His-tagged flagella as a bactria linker. 鞭毛は便宜上１本しか描いてないが実際は1~10本/細胞． |
2.CommunicationMake a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow sticky hands.  Fig2. Bacterial Communication. |
3.Size ControlMake an AHL concentration gradient for quorum sensing.  Fig3. AHL concentration gradient. |
How Our System Works
- Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Marimo. They also produce AHL to call receiver cells.
- Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders (core).
- Receivers stick to the core (the clustered senders) by way of metal ions.... one after another.
- This way, cluster and grow like a snowball. At the same time, receivers degrade AHL and thus limit the space where AHL reaches. By controlling the rate of AHL degradation, one can define the size of bacterial culster, MARIMO.
