Edinburgh/Disclaimer
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* All modelling and design work was performed by the students. | * All modelling and design work was performed by the students. | ||
* All laboratory work was performed by the students with the following exceptions: | * All laboratory work was performed by the students with the following exceptions: | ||
- | *# RT-PCR of COMT from alfalfa was attempted by Lucy Montgomery, an undergraduate Honours student who worked with the team for a part of the project period. | + | *# RT-PCR of COMT from alfalfa was attempted by '''Lucy Montgomery''', an undergraduate Honours student who worked with the team for a part of the project period. |
- | *# Attempts to transform Lactobacillus and Streptococcus (unsuccessful) and Bacillus (successful) with tpTG262-RFP were performed mainly by Chris French (Instructor). | + | *# Attempts to transform Lactobacillus and Streptococcus (unsuccessful) and Bacillus (successful) with tpTG262-RFP were performed mainly by '''Chris French''' (Instructor). |
- | *# Mutation of the PstI sites in the crtIB BioBrick,assembly of the crtEIB, lacZ-sam8 and sam8-sam5 composite BioBricks,and preliminary TLC experiments to detect vanillin pathway intermediates, were performed mainly by Chris French (Instructor). | + | *# Mutation of the PstI sites in the crtIB BioBrick,assembly of the crtEIB, lacZ-sam8 and sam8-sam5 composite BioBricks,and preliminary TLC experiments to detect vanillin pathway intermediates, were performed mainly by '''Chris French''' (Instructor). |
- | *# Initial fluorescence microscopy was performed by a colleague of Alistair Elfick (Instructor). | + | *# Initial fluorescence microscopy was performed by a colleague of '''Alistair Elfick''' (Instructor). |
In addition to our sponsors, listed above, we also gratefully acknowledge the assistance of: | In addition to our sponsors, listed above, we also gratefully acknowledge the assistance of: |
Revision as of 19:05, 26 October 2007
The Edinburgh iGEM 2007 Team declares that:
- Both projects were conceived and designed by the student team members within the time course of the project.
- All modelling and design work was performed by the students.
- All laboratory work was performed by the students with the following exceptions:
- RT-PCR of COMT from alfalfa was attempted by Lucy Montgomery, an undergraduate Honours student who worked with the team for a part of the project period.
- Attempts to transform Lactobacillus and Streptococcus (unsuccessful) and Bacillus (successful) with tpTG262-RFP were performed mainly by Chris French (Instructor).
- Mutation of the PstI sites in the crtIB BioBrick,assembly of the crtEIB, lacZ-sam8 and sam8-sam5 composite BioBricks,and preliminary TLC experiments to detect vanillin pathway intermediates, were performed mainly by Chris French (Instructor).
- Initial fluorescence microscopy was performed by a colleague of Alistair Elfick (Instructor).
In addition to our sponsors, listed above, we also gratefully acknowledge the assistance of:
- Dr. Garry Blakely, University of Edinburgh: advice on recombinases.
- Prof. Millicent Masters, University of Edinburgh: for single copy vector pHR277.
- Dr. Mike Gasson and Dr. Claire Shearman, Institute of Food Research, Norwich: for Gram positive vector pTG262.
- Dr. Anton Linnenbrink, Albert-Ludwigs-Universitat, Freiburg: for supplying DNA encoding sam8 and sam5.