Tokyo/Requirements
From 2007.igem.org
(→What is necessary for "Balanced differentiation"?) |
(→What is necessary for "Balanced differentiation"?) |
||
| Line 9: | Line 9: | ||
== What is necessary for "Balanced differentiation"? == | == What is necessary for "Balanced differentiation"? == | ||
| - | The most important and underlying point of our model is that the system is stable against environmental changes when two types of differentiated individuals coexist, called "Balanced differentiation." Our project have aimed at this "Balanced differentiation" but not dynamic equilibrium such as chemical ones. | + | The most important and underlying point of our model is that the system is stable against environmental changes when two types of differentiated individuals coexist, called "Balanced differentiation." Our project have aimed at this "Balanced differentiation" but not dynamic equilibrium such as chemical ones. |
| - | + | ||
<!--共存安定をなりたたせるために必要な要素は以下の３つである | <!--共存安定をなりたたせるために必要な要素は以下の３つである | ||
１．双安定 | １．双安定 | ||
| Line 16: | Line 15: | ||
３．２つの機能が１つの細胞から実現されること--> | ３．２つの機能が１つの細胞から実現されること--> | ||
<br>To achieve this “Balanced differentiation", our model requires | <br>To achieve this “Balanced differentiation", our model requires | ||
| - | <br>1. | + | <br>1. bistability generated by mutural-inhibition circuit. |
<br>2. cell-cell communication by quarum sensing | <br>2. cell-cell communication by quarum sensing | ||
| Line 28: | Line 27: | ||
'''~ at an individual level ~''' | '''~ at an individual level ~''' | ||
| - | For "balanced differentiation" in our model, two types of cells should coexist stably. Therefore, cells with '''THE SAME GENE SET''' need to take either of '''TWO DIFFERENTIATED STATES''', A (Worker) or B (Idler) in our project as shown in Fig. 1. '''In order to distinguish from dynamic stability, "balanced differentiation" must be achieved by two distinct states inconvertible each other.''' | + | For "balanced differentiation" in our model, two types of cells should coexist stably. Therefore, cells with '''THE SAME GENE SET''' need to take either of '''TWO DIFFERENTIATED STATES''', A (Worker) or B (Idler) in our project as shown in Fig. 1. '''In order to distinguish from dynamic stability, "balanced differentiation" must be achieved by two distinct states inconvertible each other.''' This bistability can be achieved by mutual-inhibition circuit. |
<!--[[Image:collective.jpg|thumb|350px| '''Fig.2: Coexistence]]--> | <!--[[Image:collective.jpg|thumb|350px| '''Fig.2: Coexistence]]--> | ||
Revision as of 19:26, 26 October 2007
Abstract Concept & Model Requirements Genetic_circuit Works About_our_team
What is necessary for "Balanced differentiation"?
The most important and underlying point of our model is that the system is stable against environmental changes when two types of differentiated individuals coexist, called "Balanced differentiation." Our project have aimed at this "Balanced differentiation" but not dynamic equilibrium such as chemical ones.
To achieve this “Balanced differentiation", our model requires
1. bistability generated by mutural-inhibition circuit.
2. cell-cell communication by quarum sensing
Cells with the same gene take either of two differentiated states, A (worker, red) or B (idler, green) stably.
1. Bistability
~ at an individual level ~
For "balanced differentiation" in our model, two types of cells should coexist stably. Therefore, cells with THE SAME GENE SET need to take either of TWO DIFFERENTIATED STATES, A (Worker) or B (Idler) in our project as shown in Fig. 1. In order to distinguish from dynamic stability, "balanced differentiation" must be achieved by two distinct states inconvertible each other. This bistability can be achieved by mutual-inhibition circuit.
2. Cell-cell communication
When this balanced differentiation is disrupted, by removing all the A type for example, these individual cells sense the change. Their states A or B become unstable as if cells became upset - INDIVIDUAL STATES ARE UNSTABLE ⇒ They are COLLECTIVELY UNSTABLE. Then some of them changed their states while the others do not. As a result, at a certain ratio of A and B again, the whole system comes back to a stable states. Here the cells are COLLECTIVELY STABLE ⇒ INDIVIDUALS ARE STABLE under this balanced differentiation.
Intercellular interaction is done by communication molecule AHL. When enough AHL is produced by the workers, the idlers are stable; however, if it is not ~ communication being interrupted, ~ they dedifferentiate. Thus, cells can sense the surrounding cell types.
