Melbourne/primary vf2
From 2007.igem.org
< Melbourne(Difference between revisions)
| (2 intermediate revisions not shown) | |||
| Line 4: | Line 4: | ||
*Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert | *Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert | ||
| - | + | ====Primer design and maintenance==== | |
| + | *Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry. | ||
*Primers were ordered and purchased from Sigma | *Primers were ordered and purchased from Sigma | ||
*We keep a working concentration of 10uM and a stock solution of 1mM. | *We keep a working concentration of 10uM and a stock solution of 1mM. | ||
| + | ====Problems==== | ||
| + | *Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use. | ||
| + | |||
| + | *See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details. | ||
Latest revision as of 01:50, 27 October 2007
<return to list of primary reagents> <team home page>
Contents |
Physical data
- Description: Forward primer for backbone vector
- Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert
Primer design and maintenance
- Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry.
- Primers were ordered and purchased from Sigma
- We keep a working concentration of 10uM and a stock solution of 1mM.
Problems
- Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use.
- See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details.
Lab Location
- Location: Fz1 Amp freezer west side of central lab
- Permision to use required?No
Manufacturer:
- Manufacturer:Sigma
- Manufacturers
