Melbourne/primary vf2
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====Primer design and maintenance==== | ====Primer design and maintenance==== | ||
| - | *Primers were designed identical to the [http://partsregistry.org/Part:BBa_G00100 | + | *Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry. |
*Primers were ordered and purchased from Sigma | *Primers were ordered and purchased from Sigma | ||
*We keep a working concentration of 10uM and a stock solution of 1mM. | *We keep a working concentration of 10uM and a stock solution of 1mM. | ||
Latest revision as of 01:50, 27 October 2007
<return to list of primary reagents> <team home page>
Contents |
Physical data
- Description: Forward primer for backbone vector
- Uses: Sequencing inserts into the backbone vector/colony PCR/PCR of insert
Primer design and maintenance
- Primers were designed identical to the sequence in the [http://partsregistry.org/Part:BBa_G00100 VF2 page] on the Registry.
- Primers were ordered and purchased from Sigma
- We keep a working concentration of 10uM and a stock solution of 1mM.
Problems
- Problems have been reported for these primers in which the primers are able to bind to sequences from the double terminator sequence, [http://partsregistry.org/Part:BBa_B0010 B0010], a part that we commonly use.
- See [http://partsregistry.org/Problems_with_PCR_using_VR/VF2 here] for more details.
Lab Location
- Location: Fz1 Amp freezer west side of central lab
- Permision to use required?No
Manufacturer:
- Manufacturer:Sigma
- Manufacturers
