Paris

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Latest revision as of 03:10, 27 October 2007

The SMB: Synthetic Multicellular Bacterium

The aim of our project is to engineer the first synthetic multicellular bacterium, the SMB. This new organism is a novel tool for the engineering of complex biological systems. It consists in two interdependent cell lines. The first, dedicated to reproduction is the germ line (red cells in the simulation below). It is able to differentiate into the second line: the soma (green cells), which is sterile and dedicated to support the germ line. The germ line is auxotroph for DAP (diaminopimelate) which is provided by the soma. There is thus an interdependency relationship. The soma, being sterile, requires the germ line for its generation, while the germ line needs the soma to complement its auxotrophy. We provide here both experimental and computational evidences that this system can work, as well as the almost complete construction of the SMB.

modeling <object style="width:640px;height:480px"> <param name="movie" value="http://biosynthetique.free.fr/videos/flv_player/flvplayer.swf?image=http://biosynthetique.free.fr/photo/01.jpg&autostart=false&file=http://biosynthetique.free.fr/videos/finalv4.flv" /> <param name="quality" value="high" /> </object>

For your convenience, [http://biosynthetique.free.fr/pdf/Paris_igem07.pdf download] a printable pdf version of our wiki.

Going through our wiki

Members

Student Members

Aurélien Rizk
David Bikard
David Guegan
David Puyraimond
Eimad Shotar
Fanny Caffin
Gilles Vieira
Nicolas Chiaruttini
Thomas Clozel
Thomas Landrain

Instructors

Ariel Lindner
Alfonso Jaramillo
Franck Delaplace
Francois Kepes
Vincent Schachter
Samuel Bottani

Advisors

Antoine Spicher
Francois Le Fevre
Pablo Tortosa
Maria Suarez
Serge Smidtas

Information

Site Map

Links

[http://biosynthetique.free.fr/index.php5?title=Accueil Our French wiki (SB 2006/06 journal club)]
[http://www.biologiesynthetique.fr A wiki (in french) on synthetic biology]
[http://partsregistry.org/Main_Page The registry]
[http://partsregistry.org/Help:Contents Biobricks and Registry Tutorials]
[http://en.wikipedia.org/wiki/Help:Wikitext_examples Wiki Formatting Guide]

Project

Project Description

Below, our wiki pages during work in summer 2007.

Life in the Lab

Molecular biology constructs

Team Notebook

Oligos

Protocols

Freezer

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Paris Our parts at the registry]

Forum

The team at work

From October 17th 2007:

Hit Counters

We are extremely grateful to the following organisations for their support of our project:

Fondation Bettencourt

[http://www.fondationbs.org/ www.fondationbs.org]

Sofinnova

[http://www.sofinnova.fr www.sofinnova.fr]

Ambassade de France aux Etats Unis

[http://www.ambafrance-us.org/ www.ambafrance-us.org]

Synbiocomm

[http://www.syntheticbiology.ethz.ch/synbiocomm/index www.syntheticbiology.ethz.ch]

@@ Line 1: / Line 1: @@
-<center>[[Image:Igem Paris Ban.jpg]]</center>
+[[Image:ParisLogoIGEM.jpg|350px|center]]
-more information about us:
+<font size=14><center>The SMB: Synthetic Multicellular Bacterium</center></font> <br>
-http://biosynthetique.free.fr {fr}
-<div align="center">
+<br>
-{| cellspacing="2px" cellpadding="5" border="2" style="padding: 0px; width: 780px; color: black; background-color: white;"
+{{Paris_MainMenu}}
+The aim of our project is to engineer the first synthetic multicellular bacterium, the SMB. This new organism is a novel tool for the engineering of complex biological systems. It consists in two interdependent cell lines. The first, dedicated to reproduction is the germ line (red cells in the simulation below). It is able to differentiate into the second line: the soma (green cells), which is sterile and dedicated to support the germ line. The germ line is auxotroph for DAP (diaminopimelate) which is provided by the soma. There is thus an interdependency relationship. The soma, being sterile, requires the germ line for its generation, while the germ line needs the soma to complement its auxotrophy. We provide here both experimental and computational evidences that this system can work, as well as the almost complete construction of the SMB.
+{{paris_video_modeling2}}
+For your convenience, [http://biosynthetique.free.fr/pdf/Paris_igem07.pdf download] a printable pdf version of  our wiki.
+----
+<center>'''[[Going through our wiki|Going through our wiki]]'''</center>
+<div align="center" >
+{| cellspacing="2px" cellpadding="5" border="2" style="padding: 0px; width: 780px; color: black; background-color: #708090;"
 |-valign="top"
-|width=189.25px style="padding: 10px; background-color: lightblue; border: 2px solid black;" |
+|width=189.25px style="padding: 10px; background-color: #E0FFFF; border: 2px solid black;" |
 <center><h3>Members</h3>
@@ Line 17: / Line 34: @@
 [[User:Davidoff|David Guegan]]<br>
 [[User:Davidpz|David Puyraimond]]<br>
-[[User:DrDam|Damien Robert]]<br>
 [[User:Eismoustique|Eimad Shotar]]<br>
-[[User:Fanny.c|Fanny Caffin]]<br>
+[[User:fanny.c|Fanny Caffin]]<br>
 [[User:Vieira|Gilles Vieira]]<br>
 [[User:Nicolas C.|Nicolas Chiaruttini]]<br>
-[[User:Malicia|Rayna Stamboliyska]]<br>
 [[User:Thomasclozel|Thomas Clozel]]<br>
 [[User:Landrain|Thomas Landrain]]<br>
@@ Line 33: / Line 48: @@
 [[User:Delapla|Franck Delaplace]]<br>
 [[User:Kepes|Francois Kepes]]<br>
+[[User:Vschachter|Vincent Schachter]]<br>
 [[User:Bottani|Samuel Bottani]]<br>
-[[User:Vschachter|Vincent Schachter]]<br>
 '''Advisors'''
-[[User:Chettaoui|Chafika Chettaoui|]]<br>
 [[User:Spicher|Antoine Spicher]]<br>
 [[User:flefevre|Francois Le Fevre]]<br>
 [[User:PTortosa|Pablo Tortosa]]<br>
 [[User:maria|Maria Suarez]]<br>
+[[User:SMIDTAS|Serge Smidtas]]<br>
 </center>
-|width=189.25px style="padding:10px; background-color: #ffffff; border: 2px solid black;" |
+|width=189.25px style="padding:10px; background-color: white; border: 2px solid black;" |
-<center><h3>Informations</h3></center>
+<center><h3>Information</h3></center>
-<br><br>
+'''Site Map'''
-'''[[Summary of the teachers|Summary of the teachers workshop]]  '''
+*[[Paris|Main Page]]
-<br><br>
+*[[Paris/Introduction|Introduction]]
+*[[Paris/DesignProcess |Design Process]]
+*[[Paris/Modeling|Modeling]]
+*[[Paris/ConstructionProcess |Construction Process]]
+*[[Paris/Results|Results]]
+*[[Paris/Perspectives|Perspectives]]
+*[[Paris/Acknowledgments|Acknowledgments]]
-'''Schedule'''
-* Next meeting July 19, 18h at the lab
 <br><br>
 '''Links'''
-* [http://biosynthetique.free.fr/index.php5?title=Accueil Our French wiki]
+* [http://biosynthetique.free.fr/index.php5?title=Accueil Our French wiki (SB 2006/06 journal club)]
+* [http://www.biologiesynthetique.fr A wiki (in french) on synthetic biology]
 * [http://partsregistry.org/Main_Page The registry]
 * [http://partsregistry.org/Help:Contents Biobricks and Registry Tutorials]
@@ Line 65: / Line 85: @@
 <br><br><br>
 <center>[[Image:Tour eiffel.gif|60px]]</center>
-|width=189.25px style="padding: 10px; background-color: #FF6666; border: 2px solid black;" |
+|width=189.25px style="padding: 10px; background-color: #FFA07A; border: 2px solid black;" |
 <center><h3>Project</h3></center>
+* '''[[Paris/Project Description| Project Description]]'''
+<br>
-* [[Paris/Project Description| Project Description]]
+''Below, our wiki pages during work in summer 2007.''
-* [[Paris/Modeling| Modeling]]
 '''Life in the Lab'''
+* [[Paris/Constructs| Molecular biology constructs]]
-* [[Paris/What's going on in the lab|What's going on in the lab ?]]
 * [[Paris/Notebook_Calendar| Team Notebook]]
@@ Line 84: / Line 103: @@
 * [[Paris/PROTOCOLS| Protocols]]
-* [[Paris/Fridge| Fridge]]
 * [[Paris/Freezer| Freezer]]
@@ Line 92: / Line 109: @@
-* [[Paris/DISCUSSION| Forum]] <span style='padding-left:10px; font-size: 75%;color:red'> - NEW -</span>
+* [[Paris/DISCUSSION| Forum]]
@@ Line 98: / Line 115: @@
 |}
+<html>
+From October 17th 2007:<br>
+<a href="http://www3.clustrmaps.com/counter/maps.php?url=https://2007.igem.org/Paris" id="clustrMapsLink"><img src="http://www3.clustrmaps.com/counter/index2.php?url=https://2007.igem.org/Paris" style="border:0px;" alt="Locations of visitors to this page" title="Locations of visitors to this page" id="clustrMapsImg" onError="this.onError=null; this.src='http://www2.clustrmaps.com/images/clustrmaps-back-soon.jpg'; document.getElementById('clustrMapsLink').href='http://www2.clustrmaps.com'" />
+</a>
+<br>
+<a href="http://www.easycounter.com/">
+<img src="http://www.easycounter.com/counter.php?dbikard"
+border="0" alt="Web Counters"></a>
+<br><a href="http://www.easycounter.com/FreeCounter3.html">Hit Counters</a>
+</html>
+We are extremely grateful to the following organisations for their support of our project:
+<table><tr><td>
+</td><td>
+[[Image:Paris_Sponsors.jpg]]
+</td><td>
+Fondation Bettencourt
+[http://www.fondationbs.org/ www.fondationbs.org]
+Sofinnova
+[http://www.sofinnova.fr www.sofinnova.fr]
+Ambassade de France aux Etats Unis
+[http://www.ambafrance-us.org/ www.ambafrance-us.org]
+Synbiocomm
+[http://www.syntheticbiology.ethz.ch/synbiocomm/index www.syntheticbiology.ethz.ch]
+</td></tr></table>
+[[Image:IGEMteam5.jpg|920px|center]]