Paris

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Latest revision as of 03:10, 27 October 2007

The SMB: Synthetic Multicellular Bacterium

The aim of our project is to engineer the first synthetic multicellular bacterium, the SMB. This new organism is a novel tool for the engineering of complex biological systems. It consists in two interdependent cell lines. The first, dedicated to reproduction is the germ line (red cells in the simulation below). It is able to differentiate into the second line: the soma (green cells), which is sterile and dedicated to support the germ line. The germ line is auxotroph for DAP (diaminopimelate) which is provided by the soma. There is thus an interdependency relationship. The soma, being sterile, requires the germ line for its generation, while the germ line needs the soma to complement its auxotrophy. We provide here both experimental and computational evidences that this system can work, as well as the almost complete construction of the SMB.

modeling <object style="width:640px;height:480px"> <param name="movie" value="http://biosynthetique.free.fr/videos/flv_player/flvplayer.swf?image=http://biosynthetique.free.fr/photo/01.jpg&autostart=false&file=http://biosynthetique.free.fr/videos/finalv4.flv" /> <param name="quality" value="high" /> </object>

For your convenience, [http://biosynthetique.free.fr/pdf/Paris_igem07.pdf download] a printable pdf version of our wiki.

Going through our wiki

Members

Student Members

Aurélien Rizk
David Bikard
David Guegan
David Puyraimond
Eimad Shotar
Fanny Caffin
Gilles Vieira
Nicolas Chiaruttini
Thomas Clozel
Thomas Landrain

Instructors

Ariel Lindner
Alfonso Jaramillo
Franck Delaplace
Francois Kepes
Vincent Schachter
Samuel Bottani

Advisors

Antoine Spicher
Francois Le Fevre
Pablo Tortosa
Maria Suarez
Serge Smidtas

Information

Site Map

Links

[http://biosynthetique.free.fr/index.php5?title=Accueil Our French wiki (SB 2006/06 journal club)]
[http://www.biologiesynthetique.fr A wiki (in french) on synthetic biology]
[http://partsregistry.org/Main_Page The registry]
[http://partsregistry.org/Help:Contents Biobricks and Registry Tutorials]
[http://en.wikipedia.org/wiki/Help:Wikitext_examples Wiki Formatting Guide]

Project

Project Description

Below, our wiki pages during work in summer 2007.

Life in the Lab

Molecular biology constructs

Team Notebook

Oligos

Protocols

Freezer

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=Paris Our parts at the registry]

Forum

The team at work

From October 17th 2007:

Hit Counters

We are extremely grateful to the following organisations for their support of our project:

Fondation Bettencourt

[http://www.fondationbs.org/ www.fondationbs.org]

Sofinnova

[http://www.sofinnova.fr www.sofinnova.fr]

Ambassade de France aux Etats Unis

[http://www.ambafrance-us.org/ www.ambafrance-us.org]

Synbiocomm

[http://www.syntheticbiology.ethz.ch/synbiocomm/index www.syntheticbiology.ethz.ch]

@@ Line 1: / Line 1: @@
-[[Image:ParisLogoIGEM.jpg|400px|center]]
+[[Image:ParisLogoIGEM.jpg|350px|center]]
 <font size=14><center>The SMB: Synthetic Multicellular Bacterium</center></font> <br>
@@ Line 8: / Line 8: @@
-The aim of our project is to engineer the first synthetic multicellular bacterium, the SMB. This new organism is a new tool for the engineering of complex biological systems.  It consists in two interdependent cell lines. The first one, dedicated to reproduction will be called the germ line (red cells). It is able to differentiate into the second line: the soma (green cells), which is sterile and dedicated to support the germ line. The germ line is auxotroph for DAP (diaminopimelate) which is provided by the soma. There is thus an interdependency relationship. The soma, being sterile, doesn't exist without the germ line to generate it, and the germ line needs the soma to complement its auxotrophy. We provide here experimental and computational evidences that this system can work, as well as the almost complete construction of the SMB.
+The aim of our project is to engineer the first synthetic multicellular bacterium, the SMB. This new organism is a novel tool for the engineering of complex biological systems. It consists in two interdependent cell lines. The first, dedicated to reproduction is the germ line (red cells in the simulation below). It is able to differentiate into the second line: the soma (green cells), which is sterile and dedicated to support the germ line. The germ line is auxotroph for DAP (diaminopimelate) which is provided by the soma. There is thus an interdependency relationship. The soma, being sterile, requires the germ line for its generation, while the germ line needs the soma to complement its auxotrophy. We provide here both experimental and computational evidences that this system can work, as well as the almost complete construction of the SMB.
-<br>
 {{paris_video_modeling2}}
-<br>
+For your convenience, [http://biosynthetique.free.fr/pdf/Paris_igem07.pdf download] a printable pdf version of  our wiki.
+----
+<center>'''[[Going through our wiki|Going through our wiki]]'''</center>
 <div align="center" >
-{| cellspacing="2px" cellpadding="5" border="2" style="padding: 0px; width: 780px; color: black; background-color: white;"
+{| cellspacing="2px" cellpadding="5" border="2" style="padding: 0px; width: 780px; color: black; background-color: #708090;"
 |-valign="top"
-|width=189.25px style="padding: 10px; background-color: lightblue; border: 2px solid black;" |
+|width=189.25px style="padding: 10px; background-color: #E0FFFF; border: 2px solid black;" |
 <center><h3>Members</h3>
@@ Line 30: / Line 35: @@
 [[User:Davidpz|David Puyraimond]]<br>
 [[User:Eismoustique|Eimad Shotar]]<br>
+[[User:fanny.c|Fanny Caffin]]<br>
 [[User:Vieira|Gilles Vieira]]<br>
 [[User:Nicolas C.|Nicolas Chiaruttini]]<br>
@@ Line 47: / Line 53: @@
 '''Advisors'''
-[[User:Chettaoui|Chafika Chettaoui|]]<br>
 [[User:Spicher|Antoine Spicher]]<br>
 [[User:flefevre|Francois Le Fevre]]<br>
@@ Line 56: / Line 61: @@
 </center>
-|width=189.25px style="padding:10px; background-color: #ffffff; border: 2px solid black;" |
+|width=189.25px style="padding:10px; background-color: white; border: 2px solid black;" |
-<center><h3>Informations</h3></center>
+<center><h3>Information</h3></center>
-<br><br>
+'''Site Map'''
-'''[[Summary of the teachers|Summary of the teachers workshop]]  '''
+*[[Paris|Main Page]]
-<br><br>
+*[[Paris/Introduction|Introduction]]
+*[[Paris/DesignProcess |Design Process]]
+*[[Paris/Modeling|Modeling]]
+*[[Paris/ConstructionProcess |Construction Process]]
+*[[Paris/Results|Results]]
+*[[Paris/Perspectives|Perspectives]]
+*[[Paris/Acknowledgments|Acknowledgments]]
-'''Schedule'''
-* '''Next meeting : Wednesday october 24th'''
-Results, Conclusions, Wiki
-Wiki freezing:  D-5 !!
 <br><br>
 '''Links'''
 * [http://biosynthetique.free.fr/index.php5?title=Accueil Our French wiki (SB 2006/06 journal club)]
+* [http://www.biologiesynthetique.fr A wiki (in french) on synthetic biology]
 * [http://partsregistry.org/Main_Page The registry]
 * [http://partsregistry.org/Help:Contents Biobricks and Registry Tutorials]
@@ Line 77: / Line 85: @@
 <br><br><br>
 <center>[[Image:Tour eiffel.gif|60px]]</center>
-|width=189.25px style="padding: 10px; background-color: #FF6666; border: 2px solid black;" |
+|width=189.25px style="padding: 10px; background-color: #FFA07A; border: 2px solid black;" |
 <center><h3>Project</h3></center>
 * '''[[Paris/Project Description| Project Description]]'''
+<br>
+''Below, our wiki pages during work in summer 2007.''
@@ Line 144: / Line 154: @@
 [http://www.syntheticbiology.ethz.ch/synbiocomm/index www.syntheticbiology.ethz.ch]
 </td></tr></table>
+[[Image:IGEMteam5.jpg|920px|center]]