Calgary/final result
From 2007.igem.org
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<p>At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present. This section summarizes the final results of our work with <em>E. co</em>Lisa.</p> | <p>At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present. This section summarizes the final results of our work with <em>E. co</em>Lisa.</p> | ||
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<td valign="top" width="25%"><p><a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#contributions" title="See our contributions to the Registry" >Contributions To The Registry</a></td> | <td valign="top" width="25%"><p><a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#contributions" title="See our contributions to the Registry" >Contributions To The Registry</a></td> | ||
<td valign="top" width="25%"><p><a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#agarase" title="Sequencing of Agarase" >Sequencing of Agarase</a></td> | <td valign="top" width="25%"><p><a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#agarase" title="Sequencing of Agarase" >Sequencing of Agarase</a></td> | ||
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<td valign="top" width="25%"><p> <a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#logic" title="Sequencing of Logic Circut" >Sequencing of Logic Circut </a></td> | <td valign="top" width="25%"><p> <a style="font-size:18px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#logic" title="Sequencing of Logic Circut" >Sequencing of Logic Circut </a></td> | ||
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| - | <p style="margin-bottom: | + | <p style="margin-bottom:40px">Our team is pleased to report that we have submitted 13 unique parts to the iGEM registry. These parts include the β-agarase gene, our logic circut and several of the intermediate parts we constructed. </p> |
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We were able to sucessfully isolate β-agarase gene from the <em>Pseudoalteromonas atlantica</em> genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format. | We were able to sucessfully isolate β-agarase gene from the <em>Pseudoalteromonas atlantica</em> genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format. | ||
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<a href="https://static.igem.org/mediawiki/2007/8/85/Agaraseclone_sequence.pdf" target="_blank"><img style="border:#CCCCCC thin outset" src="https://static.igem.org/mediawiki/2007/9/9f/AgaraseSequencingThumb.gif" atl="sequencing information from β-agarase gene" /></a> | <a href="https://static.igem.org/mediawiki/2007/8/85/Agaraseclone_sequence.pdf" target="_blank"><img style="border:#CCCCCC thin outset" src="https://static.igem.org/mediawiki/2007/9/9f/AgaraseSequencingThumb.gif" atl="sequencing information from β-agarase gene" /></a> | ||
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| + | The sequencing information obtained confirms that we have indeed isolated the β-agarase gene and it is present in the plasmid. | ||
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| + | <img src="https://static.igem.org/mediawiki/2007/b/b6/AgaraseChromatogramForward.jpg" alt="Reverse Chromatogram from aragase" /> | ||
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| + | This is the Reverse Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid</p> | ||
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| + | <img src="https://static.igem.org/mediawiki/2007/7/7a/AgaraseChromatogramForward2%28actuallyForward%29.jpg" atl="Forward Chromatogram from agarase" /> | ||
| + | </td> | ||
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| + | This is the Forward Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid</p> | ||
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| + | <td style="width:85%;"><p style="font-size:20px; font-weight:bold;"><a style="text-decoration:none" name="logic"> Sequencing of Logic Circut</a></p></td> | ||
| + | <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td> | ||
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| + | </table> | ||
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| + | There were three seperate logic ciructs prepared. Each differed only in the initial promotorer being used. The first circut used part R0084 a promter that is activated by phosphorylated <em>ompR</em> while the other two R0082 and R0083 are repressed by phosphorylated <em>ompR</em> | ||
| + | </p> | ||
| + | <p> Our team synthesized plasmids containing our logic circut using each of these promoters. The links below connect to pdf's showing the result of the sequencing for each of these plasmids. The sequencing information confirmed that our logic circut was present in the plasmids. | ||
| + | </p> | ||
| + | |<a href="https://static.igem.org/mediawiki/2007/5/55/R82akgv.pdf" title="R0082 Logic Circut">R0082 Logic Circut</a>| |<a href="https://static.igem.org/mediawiki/2007/3/3a/R83AKVF5FR83AKVR.pdf" title="R0083 Logic Circut">R0083 Logic Circut</a>| |<a href="https://static.igem.org/mediawiki/2007/a/aa/R84AKGB.pdf" title="R0084 Logic Circut">R0084 Logic Circut</a>| | ||
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Revision as of 03:40, 27 October 2007
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| Projects | Design: Wet Lab | Design: Printer | Design: Software | Testing | Construction: The Wetlab | Protocols | Final Result of E.co Lisa |
At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present. This section summarizes the final results of our work with E. coLisa.
| return to top |
| Number | Parts | Plasmid | New part name | Type | Notes |
| 1 | agarase | pSB1A2 | i737020 | Basic Part | |
| 2 | R0084 . A340620 . S01414 . I13504 | pSB1AK3 | I737010 | Project | |
| 3 | R0083 . A340620 . S01414 . I13504 | pSB1AK3 | I737012 | Project | |
| 4 | R0082 . A340620 . S01414 . I13504 | pSB1AK3 | I737014 | Project | |
| 5 | R0040 . A340620 . I13504 | pSB1AC3 | S03868 | Intermediate | Entered as part I13553 and J26010, not available or experience |
| 6 | R0040 . J01010 . I13401 | pSB1AC3 | S03867 | Intermediate | Entered as part I714081, but not available |
| 7 | R0040 . J01008 . B0015 | pSB1AC3 | S03863 | Intermediate | Is S03862 . B0015 |
| 8 | R0040 . J01080 . I13401 | pSB1AC3 | S03864 | Intermediate | Is J01109 . I13401 |
| 9 | R0040 . J23008 . B0015 | pSB1AC3 | S03866 | Intermediate | Is S03865 . B0015 |
| 10 | R0040 . J01010 . E0040 . S01414 . B0015 | pSB1AK3 | S03871 | Intermediate | Is S03870 . I0462 |
| 11 | S01414 . I13504 | pSB1AK3 | S03869 | Intermediate | |
| 12 | R0040 . A340620 . J23008 . B0015 | pSB1AK3 | S03872 | Intermediate | Is F2620 . J23021 |
| 13 | R0040 . J06801 | pSB2K3 | S03743 | Intermediate |
Our team is pleased to report that we have submitted 13 unique parts to the iGEM registry. These parts include the β-agarase gene, our logic circut and several of the intermediate parts we constructed.
| return to top |
We were able to sucessfully isolate β-agarase gene from the Pseudoalteromonas atlantica genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format.
|
The sequencing information obtained confirms that we have indeed isolated the β-agarase gene and it is present in the plasmid. |
|
This is the Reverse Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid |
|
This is the Forward Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid |
| return to top |
There were three seperate logic ciructs prepared. Each differed only in the initial promotorer being used. The first circut used part R0084 a promter that is activated by phosphorylated ompR while the other two R0082 and R0083 are repressed by phosphorylated ompR
Our team synthesized plasmids containing our logic circut using each of these promoters. The links below connect to pdf's showing the result of the sequencing for each of these plasmids. The sequencing information confirmed that our logic circut was present in the plasmids.
|R0082 Logic Circut| |R0083 Logic Circut| |R0084 Logic Circut|