Edinburgh/DivisionPopper/Conclusions

From 2007.igem.org

(Difference between revisions)
Line 2: Line 2:
-
We have designed a signal output device that works as a function of bacterial division. The PoPper is naturally oscillatory, as opposed to other engineered devices. Output frequency depends on what strain you place the device in. The ''dif'' sites are recombinatorial solutions that are employed universally by bacteria, so the PoPper should function well outwith the ''E. coli'' scope.
+
We have designed a device called Division PoPer that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted in the bacteria.
-
 
+
We designed and implemented it by following the paradigms of Synthetic Biology approach and we designed also a Proof of Concept device for proving the validity of the main assumptions and mechanisms.
-
The Divison PoPper has been a collected team effort. At the time of writing, we're still waiting for the Division Popper to arrive from GeneArt. Delivery has now been delayed by over a month and it looks like final piece in this jigsaw puzzle will be left out for now. The project does not end here, but will be continued as a final year dissertation project by a student, but we would have liked to present results.
+
The most important mechanism is related to the dif sites, ecombinatorial solutions that are employed universally by bacteria.
-
 
+
We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.
-
All the research we did into the topic still allows us to visualise the system outcome. The computer models show our predictions.
+
-
 
+
-
This wiki will be updated until the very moment it freezes, so please watch this space. We keep our fingers crossed that the construct arrives soon.
+
-
 
+
-
 
+
-
 
+
-
[[Image:Popper.JPG]]
+
-
 
+
-
Please take some time to visit [http://www.macteria.co.uk/popper.php This page ] for an interactive Flash animation of the final construct!
+
We look forward to meeting you all at the Jamboree.
We look forward to meeting you all at the Jamboree.

Revision as of 04:28, 27 October 2007

MENU : Introduction | Background | Applications | Design&Implementation | Modelling | Wet Lab | Synthetic Biology Approach | Conclusions


We have designed a device called Division PoPer that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted in the bacteria. We designed and implemented it by following the paradigms of Synthetic Biology approach and we designed also a Proof of Concept device for proving the validity of the main assumptions and mechanisms. The most important mechanism is related to the dif sites, ecombinatorial solutions that are employed universally by bacteria. We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.

We look forward to meeting you all at the Jamboree.

Retrieved from "http://2007.igem.org/wiki/index.php/Edinburgh/DivisionPopper/Conclusions"