Edinburgh/DivisionPopper/Conclusions

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'''MENU''' :[[Edinburgh/DivisionPopper| Introduction]] | [[Edinburgh/DivisionPopper/References|Background]] | [[Edinburgh/DivisionPopper/Applications|Applications]] | [[Edinburgh/DivisionPopper/Design|Design]] | [[Edinburgh/DivisionPopper/Modelling|Modelling]] | [[Edinburgh/DivisionPopper/Status|Status]] | [[Edinburgh/DivisionPopper/SBApproach|Synthetic Biology Approach]] | [[Edinburgh/DivisionPopper/Conclusions|Conclusions]]  
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'''MENU''' :[[Edinburgh/DivisionPopper| Introduction]] | [[Edinburgh/DivisionPopper/References|Background]] | [[Edinburgh/DivisionPopper/Applications|Applications]] | [[Edinburgh/DivisionPopper/Design|Design&Implementation]] | [[Edinburgh/DivisionPopper/Modelling|Modelling]] | [[Edinburgh/DivisionPopper/Status|Wet Lab]] | [[Edinburgh/DivisionPopper/SBApproach|Synthetic Biology Approach]] | [[Edinburgh/DivisionPopper/Conclusions|Conclusions]]
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Our team thinks of iGEM as a competition with two goals: the design and construction of a genetically engineered machine that proves to be useful and the investigation and innovation of the Synthetic Biology theoretical and practical tools.
 
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Because of that, we spent as much attention and time in applying the Synthetic Biology paradigms and guidelines as in selecting and developing the actual construct. Since Synthetic Biology is a complex but quite new field of research, we think that even a group of undergraduate/graduate students working for two months on the project can help in investigating small innovations or improving the standard approach. In this section we explain the work we did in this direction.
 
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== Synthetic Biology Approach ==
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We have designed a device called Division PoPper that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria.
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We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms.
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The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria.
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We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device.
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In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry. We propose our device as interesting example of conversion from a meaningful biological process to a standard logical signal. The device can be used for controlling cell division frequency or trigger actions related to division counting.
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As our understanding, Synthetic Biology aims to bring in the field of bioengineering the that belongs to engineering field.  
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We look forward to meeting you all at the Jamboree.
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=== Abstraction ===
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=== Decoupling ===
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=== Standards ===
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=== Compositionality ===
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== Investigating Innovations ==
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=== Stochastic Modelling with Process Algebras ===
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=== Representing biological processes with standard notations ===
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Latest revision as of 05:00, 27 October 2007

MENU : Introduction | Background | Applications | Design&Implementation | Modelling | Wet Lab | Synthetic Biology Approach | Conclusions


We have designed a device called Division PoPper that works as a function of bacterial division. The device reports when a bacterial division happens by generating a PoPS pulse on a single copy plasmid inserted into the bacteria. We designed and implemented it by following the paradigms of Synthetic Biology approach and we also designed a Proof of Concept device for proving the validity of the main assumptions and mechanisms. The most important mechanism to test is related to the dif sites, recombinatorial solutions that are employed universally by bacteria. We modelled both devices using classical and stochastic techniques and showed the quantitative and qualitative possibility of costructing such devices and composing it with a counter device. In the wet lab we designed and constructed a total of thirteen biobricks that we registered and commented in the Registry. We propose our device as interesting example of conversion from a meaningful biological process to a standard logical signal. The device can be used for controlling cell division frequency or trigger actions related to division counting.

We look forward to meeting you all at the Jamboree.

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