Chiba/Communication

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Revision as of 05:04, 27 October 2007

Communication Unit

Our Aim

Fig13. Quorum sensing for activating receivers!

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig14. Constructed circuit of Quorum sensing in our system.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.

Detailed gene circuit is discribed in Fig. 2.

• more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments: New Parts!!

Recievers

New Parts We Made!!

• AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
  • 
• AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
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• Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]

From Biobrick

• BBa_T9002
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Future Work

• inverter-aiiA
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Senders

New Parts We Made

• MetK Sender ( could not deposit to registry )

From Biobrick

• Sender(BBa_S03623)