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-	Preliminary assays	+	__NOTOC__
-		+	<br>[[Tokyo/Works|Works top]]&nbsp;&nbsp;&nbsp;0.[[Tokyo/Works/Hybrid promoter|Hybrid promoter]]&nbsp;&nbsp;&nbsp;1.[[Tokyo/Works/Formulation |Formulation]]　　'''2.[[Tokyo/Works/Assay |Assay1]]'''&nbsp;&nbsp;&nbsp;3.[[Tokyo/Works/Simulation |Simulation]]&nbsp;&nbsp;&nbsp;4.[[Tokyo/Works/Assay2 |Assay2]]&nbsp;&nbsp;&nbsp;5.[[Tokyo/Works/Future works |Future works]]
		+	<br><br>
		+	[[Tokyo/Hill function fitting |Objective of this assay]]&nbsp;&nbsp;&nbsp;[[Tokyo/AHL assay|Effect of AHL]]&nbsp;&nbsp;&nbsp;[[Tokyo/IPTG assay|Effect of LacI-IPTG]]　　[[Tokyo/Preliminary assays |Preliminary assays]]
		+	== ==
		+	'''Preliminary assays were conducted'''
		+	<br>- '''to practice and experience the assays with AHL and IPTG'''
		+	<br>- '''to check if and how our materials actually worked.'''
	== [[Tokyo/AHL preparation |AHL preparation]] ==		== [[Tokyo/AHL preparation |AHL preparation]] ==
		+	== [[Tokyo/Experimental precision and accurecy|Experimental precision and accuracy]] ==
		+	== [[Tokyo/AHL assay practice|AHL assay practice]] ==
		+	== [[Tokyo/Lux-lac hybrid promoter activation check|Lux-lac hybrid promoter activation check]] ==
		+	== [[Tokyo/OD correction|OD correction]] ==
		+	== [[Tokyo/Wash|Wash]] ==
		+	== [[Tokyo/New AHL check|New AHL check]] ==
		+	<!--== [[Tokyo/Hybrid promoter activation & repression check|Hybrid promoter activation & repression check ]] ==
-	== OD correction ==	+	<br>
-		+	-->
-	===Date: ===	+
-	2007/09/21	+
-	===Purpose: ===	+
-	===Samples: ===	+
-	===Procedure: ===	+
-	===Result: ===	+
-	===Conclusion: ===	+
-		+
-		+
-	== Wash ==	+
-		+
-	===Date: ===	+
-	20070925	+
-	===Purpose: ===	+
-	===Samples: ===	+
-	===Procedure: ===	+
-	===Result: ===	+
-	===Conclusion: ===	+
-		+
-		+
-	== Detailed AHL assay ==	+
-		+
-	===Date: ===	+
-	20070925	+
-	===Purpose: ===	+
-	===Samples: ===	+
-	===Procedure: ===	+
-	===Result: ===	+
-	===Conclusion: ===	+
-		+
-	== New-AHL assay ==	+
-		+
-	===Date: ===	+
-	20070926	+
-	===Purpose: ===	+
-	===Samples: ===	+
-	===Procedure: ===	+
-	===Result: ===	+
-	===Conclusion: ===	+
-		+
-	== Lux-lac hybrid promoter Check ==	+
-		+
-	[[Image:luxlachybrid.jpg]]	+
-		+
-	=== Date: ===	+
-	2007/09/20, 21	+
-		+
-	===Purpose 1:===	+
-	To check if LacI hybrid promoter is activated by AHL and repressed by LacI.	+
-	===Purpose 2:===	+
-	To obtain parameters of LacI hybrid promoter for computational simulation.	+
-		+
-	===Samples: ===	+
-	<br>1. pTrc99A + [LacI hybrid promoter – GFP] (LacI+)	+
-	<br>2. pBR322 TetR + [LacI hybrid promoter – GFP] (lacI-)	+
-	<br>3. pTrc99A + [placQI – GFP] (placQI is constitutive promoter)	+
-	<br>4. pTrc322 TetR + [LacI promoter – GFP]	+
-	<br>5. pBR322 TetR + [LacI promoter – GFP]	+
-		+
-	<br>'''Repressor LacI expression:'''	+
-	<br>pTrc99A expresses LacI	+
-	<br>pBR322 TetR does NOT express LacI	+
-		+
-	<br>'''Antibiotics resistance:'''	+
-	<br>pTrc99A gives ampicillin-resistance	+
-	<br>pBR322 TetR gives ampicillin-resistance	+
-	<br>[LacI hybrid promoter – GFP] gives kanamicin-resistance	+
-	<br>[placQI – GFP] gives kanamicin-resistance	+
-		+
-	===Procedure:===	+

---

Latest revision as of 05:12, 27 October 2007

Works top   0.Hybrid promoter   1.Formulation　　2.Assay1   3.Simulation   4.Assay2   5.Future works

Objective of this assay   Effect of AHL   Effect of LacI-IPTG　　Preliminary assays

Preliminary assays were conducted
- to practice and experience the assays with AHL and IPTG
- to check if and how our materials actually worked.

AHL preparation

Experimental precision and accuracy

AHL assay practice

Lux-lac hybrid promoter activation check

OD correction

Wash

New AHL check