Chiba/Project Design

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==Project Design==
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<html><link rel="stylesheet" href="/igem07/index.php?title=User:Maiko/Chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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Project design for iGEM 2007!
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[[Image:chiba_logo.png|center]]
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__NOTOC__
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{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
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| align="center" |
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[[Chiba|Home]]<br>
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<span style="font-size:120%;font-weight:bold;">[[Chiba/Introduction|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) |  [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]]</span><br>
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[[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]]
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|}
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===Marimo Bacteria===
 
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*[[user:Hiroki|Hiroki Fukutomi(福冨　浩樹)]]
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==Project Design==
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[[Image:Igem marimo2.jpg|350x248px]]
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===Concept===
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[[Image:Story.PNG|frame|'''Fig. 4''' Concept|center]]<br>
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鞭毛同士をつなげて'''まりも状の立体的な集合体をつくる'''こと、'''ある程度の大きさになったところで集合体の成長が止まる'''ことが目標
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We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.
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AHLの濃度勾配を使って大きさを感知させる
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さらに応用として、縁取りお絵描きをすることにも使えそう
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===What our system requires===
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{| style="border:0px;" cellpadding="5px"
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| width="30%" valign="top" |
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====[[Chiba/Engeneering_Flagella|1.Affinity Tag]]====
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Make a '''His-tagged Flagella'''. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.<br>
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詳しくは上のポスターを見てください（クリックで拡大します）
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[[Image:Chiba_stickbacteria.png|frame|left|'''Fig. 5 ''' His-tagged flagella as a bactria linker. This image depicts only one tail as flagella (the real bacteria have about ten flagella per cell).]]<br>
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| width="30%" valign="top" |
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====[[Chiba/Communication|2.Communication Module]]====
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Make a '''Bacterial Communication'''. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.<br>
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===Gangway!Gangway!Bacteria===
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[[Image:Chiba_prj_com.png|frame|left|'''Fig. 6''' Bacterial Communication.]]
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| width="30%" valign="top" |
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*[[user:Yukihiro|Yukihiro Tkami(高見　行洋）]]
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====[[Chiba/Quorum_Sensing|3.Size Control]]====
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Make an '''AHL localized region''' for quorum sensing.
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[[Image:Chiba_proj_ahlconc.png|frame|left|'''Fig. 7''' Controlling AHL diffusing area and the size of Bacteria Marimo.]]
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|}
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大腸菌の忌避物質であるindoleを多量に生産し、自分たち以外の大腸菌をどかしてしまう、そんな嫌なやつをつくることが目標
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===How Our System Works===
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[[Image:How our system works chiba.PNG|frame|center|'''Fig. 8''' How Our System Works.]]<br>
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#Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Bacteria Marimo. The senders also produce AHL to sign the receiver cells.<br>
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#Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders core.<br>
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#Receivers stick to the senders core and themselves through metal ions.... one after another.<br>
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#As seen, the cluster grows like a snowball. At the same time, the receivers degrade AHL and thus AHL diffusion space around the mixed cluster limited. By controlling the rate of AHL degradation and so on, one can define the size of such Bacteria Marimo.<br>
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indoleを感じて働くprmoterがないため、逃げる側は最初から緑色にしようと思ってます。詳しくはpdfを見てください。
 
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　pdf ファイルはこちら→[[https://static.igem.org/mediawiki/2007/6/67/Dokeodke.pdf]]
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'''For other strategies to make marimo, visit the official website of [http://chem.tf.chiba-u.jp/igem/ iGEMCHIBA]'''
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<!-- SenderやReceiverの色を対応させてみたらどう？　ｂｙ田代 -->

Latest revision as of 05:36, 27 October 2007

Chiba logo.png

Home
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿


Project Design

Concept

Fig. 4 Concept

We aimed to make a spherical gathering of bacteria such like marimo by ordering bacteria go get together and stick to each other.

What our system requires

1.Affinity Tag

Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.

Fig. 5 His-tagged flagella as a bactria linker. This image depicts only one tail as flagella (the real bacteria have about ten flagella per cell).

2.Communication Module

Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.

Fig. 6 Bacterial Communication.

3.Size Control

Make an AHL localized region for quorum sensing.

Fig. 7 Controlling AHL diffusing area and the size of Bacteria Marimo.

How Our System Works

Fig. 8 How Our System Works.

  1. Senders whose flagella display the histidine tags stick to each other through metal ions. This becomes the core of Bacteria Marimo. The senders also produce AHL to sign the receiver cells.
  2. Receivers express his/flagella and GFP in high [AHL]; only when they get close to the senders core.
  3. Receivers stick to the senders core and themselves through metal ions.... one after another.
  4. As seen, the cluster grows like a snowball. At the same time, the receivers degrade AHL and thus AHL diffusion space around the mixed cluster limited. By controlling the rate of AHL degradation and so on, one can define the size of such Bacteria Marimo.


For other strategies to make marimo, visit the official website of [http://chem.tf.chiba-u.jp/igem/ iGEMCHIBA]

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