Chiba/Communication

From 2007.igem.org

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[[Image:chiba_logo.png|center]]
[[Image:chiba_logo.png|center]]
__NOTOC__
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{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
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[[Chiba|Introduction]] | [[Chiba/Project_Design|Project Design]] | [[Chiba/Engeneering_Flagella|Bacteria Linker]] | [[Chiba/Communication|Communication]] | [[Chiba/Quorum_Sensing|Size Controller]] | [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]] || [[Chiba/Team_Members|Team Members]] | [[Chiba/Members_Only|メンバ連絡簿]]
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[[Chiba|Home]]<br>
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<span style="font-size:120%;font-weight:bold;">[[Chiba/Introduction|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) | [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]]</span><br>
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[[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]]  
|}
|}
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==Communication: Our Aim==
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==Communication Unit==
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[[Image:Chiba_prj_com.png|frame|'''Fig1.''']]
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===Our Aim===
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違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてstickey handsを生やす．そして合体．このシグナルにはQuorum sensingを利用する．
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[[Image:Chiba_prj_com.png|frame|'''Fig. 13''' Quorum sensing for activating receivers!]]
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Our aim is to make bacteria stick around a bacteria core.
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Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
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Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
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Then they stick together. We use Quorum sensing as a signal.
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==About Quorum Sensing==
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===About Quorum Sensing===
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[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']]
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[[Image:Chiba_aboutqs.png|frame|'''Fig. 14''' Constructed circuit of Quorum sensing in our system.]]
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クオラムセンシングとは，バクテリアが細胞間で行う（細胞密度に依存する）シグナル伝達のシステムである（＜この辺wikipediaから引用する）．自然界ではバクテリア（限定したほうが良い？）は常にAHLを合成している．AHLの濃度がある値以上になる，すなわち細胞密度がある値を超えるとAHL依存性プロモータ（LuxP）のスイッチがオンになり，遺伝子が発現される．
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Quorum sensing is a cell-to-cell signaling action of bacteria.
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詳しい遺伝子回路は図に示す通り．
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They detect the cell density of the same species and coordinate the expression behavior of their cells.
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Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
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Detailed gene circuit is discribed in Fig. 2.
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*more about quorum sensing
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[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
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==Our Construct==
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[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
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==Experiments: New Parts!!==
===Recievers===
===Recievers===
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*ver1.
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====New Parts We Made!!====
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**ptet-luxr-plux-gfp-aiia
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*AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
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*ver2.
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**[[Image:BBa I729006 circuit.jpg]]
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**ptet-luxr-plux-gfp-inv-aiia
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*AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
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===Sender===
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**[[Image:BBa I729005 circuit.jpg]]
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*ptet-luxI( (use BBa_...)
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*Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
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**[[Image:Chiba_BBa_I729004.png]]
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====From Biobrick====
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*BBa_T9002
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**[[Image:T9002 circuit.jpg]]
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====Future Work====
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*inverter-aiiA
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**[[Image:Rec inv aiia.jpg|357x40px]]
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===Senders===
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====New Parts We Made====
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*MetK Sender ( could not deposit to registry; sequencing half completed )
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[[Image:MetK circuit.jpg]]
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====From Biobrick====
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*Sender(BBa_S03623)
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[[Image:BBa s03623.jpg]]
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<!--
==Experiments==
==Experiments==
===Sender Assay===
===Sender Assay===
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*調べる the difference in synthesis ability among Strains.
*調べる the difference in synthesis ability among Strains.
*調べるwhether does it change or not when the AHL precursur, methionine, is added. Right we show the AHL synthesis pathway.
*調べるwhether does it change or not when the AHL precursur, methionine, is added. Right we show the AHL synthesis pathway.
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====Procedure====
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====Procedure and result====
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[[Image:Chiba_senderassay.jpg]]
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c.f.[[Chiba/Quorum Sensing#1.Improving Sender|Improving Sender]]
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====Result====
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[[Image:AHL_test_photo.jpg]]
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1.Senderは少なくとも5x10^4は必要である。
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2.Senderの株によってLuxIの活性が違う。
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-->

Latest revision as of 05:37, 27 October 2007

Chiba logo.png

Home
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿


Communication Unit

Our Aim

Fig. 13 Quorum sensing for activating receivers!

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig. 14 Constructed circuit of Quorum sensing in our system.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.

Detailed gene circuit is discribed in Fig. 2.

  • more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments: New Parts!!

Recievers

New Parts We Made!!

  • AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
    • BBa I729006 circuit.jpg
  • AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
    • BBa I729005 circuit.jpg
  • Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
    • Chiba BBa I729004.png

From Biobrick

  • BBa_T9002
    • T9002 circuit.jpg

Future Work

  • inverter-aiiA
    • Rec inv aiia.jpg

Senders

New Parts We Made

  • MetK Sender ( could not deposit to registry; sequencing half completed )

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg

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