Chiba/Communication

From 2007.igem.org

< Chiba(Difference between revisions)
(→About Quorum Sensing)
(→New Parts We Made)
 
(25 intermediate revisions not shown)
Line 2: Line 2:
[[Image:chiba_logo.png|center]]
[[Image:chiba_logo.png|center]]
__NOTOC__
__NOTOC__
-
{| style="border:0;width:100%;" cellpadding="20px" cellspacing="0"
+
{| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
| align="center" |
| align="center" |
-
[[Chiba|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Sticky Hands]] | [[Chiba/Communication|2.Communication]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) |  [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]] || [[Chiba/Team_Members|Team Members]] | [[Chiba/Members_Only|メンバ連絡簿]]
+
[[Chiba|Home]]<br>
 +
<span style="font-size:120%;font-weight:bold;">[[Chiba/Introduction|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) |  [[Chiba/Making Marimo|Making Marimos]] |  [[Chiba/Goal|Our Goal]]</span><br>
 +
[[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]]  
|}
|}
-
==Communication: Our Aim==
+
==Communication Unit==
-
[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]]
+
===Our Aim===
-
違う遺伝子回路を持つ２つの細胞（sender&receiver）に分ける．あらかじめお互い結合しているSenderが，周りのreceiverにシグナルを与えてstickey handsを生やす．そして合体．このシグナルにはQuorum sensingを利用する．
+
[[Image:Chiba_prj_com.png|frame|'''Fig. 13''' Quorum sensing for activating receivers!]]
 +
Our aim is to make bacteria stick around a bacteria core.
 +
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
 +
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
 +
Then they stick together. We use Quorum sensing as a signal.
<br clear="all">
<br clear="all">
-
==About Quorum Sensing==
+
===About Quorum Sensing===
-
[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']]
+
[[Image:Chiba_aboutqs.png|frame|'''Fig. 14''' Constructed circuit of Quorum sensing in our system.]]
-
Quorum Sinsing is cell-to-cell signaling action of bacteria.
+
Quorum sensing is a cell-to-cell signaling action of bacteria.
-
Detect the cell density of same species and coordinating the behavior of cells within a population.
+
They detect the cell density of the same species and coordinate the expression behavior of their cells.
-
Species of Gram-Negativfe signaling information transfer molecule (autoinducer) is acyl homoserine lactone (AHL).When AHL concentration exceed threshold thus the cell population over a certain number, AHL regulated promoter is activated transcription.
+
Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
-
クオラムセンシングとは，バクテリアが細胞間で行うシグナル伝達のシステムである．自分と同種の菌の生息密度を感知して、それに応じて物質の産生をコントロールする．情報伝達に使用している化合物（オートインデューサー）はグラム陰性菌の場合AHLである．AHLの濃度がある値以上になる，すなわち細胞密度がある値を超えるとAHL依存性プロモータ（LuxP）のスイッチがオンになり，遺伝子が発現される．
+
-
詳しい遺伝子回路は図に示す通り．
+
-
<br clear="all">
+
 +
Detailed gene circuit is discribed in Fig. 2.
 +
<br clear="all">
*more about quorum sensing
*more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
Line 27: Line 32:
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
-
==Our Construct==
+
==Experiments: New Parts!!==
===Recievers===
===Recievers===
-
====We Made====
+
====New Parts We Made!!====
-
*ver.1(BBa_T729006)
+
*AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
**[[Image:BBa I729006 circuit.jpg]]
**[[Image:BBa I729006 circuit.jpg]]
-
*ver2.(BBa_I729005)
+
*AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
**[[Image:BBa I729005 circuit.jpg]]
**[[Image:BBa I729005 circuit.jpg]]
 +
*Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
 +
**[[Image:Chiba_BBa_I729004.png]]
 +
====From Biobrick====
====From Biobrick====
-
*ver.0(BBa_T9002)
+
*BBa_T9002
**[[Image:T9002 circuit.jpg]]
**[[Image:T9002 circuit.jpg]]
====Future Work====
====Future Work====
-
*ver3
+
*inverter-aiiA
**[[Image:Rec inv aiia.jpg|357x40px]]
**[[Image:Rec inv aiia.jpg|357x40px]]
-
===Sender===
+
===Senders===
-
====We Made====
+
====New Parts We Made====
-
*MetK Sender
+
*MetK Sender ( could not deposit to registry; sequencing half completed )
[[Image:MetK circuit.jpg]]
[[Image:MetK circuit.jpg]]
 +
====From Biobrick====
====From Biobrick====
*Sender(BBa_S03623)
*Sender(BBa_S03623)
[[Image:BBa s03623.jpg]]
[[Image:BBa s03623.jpg]]
-
 
+
<!--
-
futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
+
-
 
+
==Experiments==
==Experiments==
===Sender Assay===
===Sender Assay===
Line 62: Line 69:
  1.Senderは少なくとも5x10^4は必要である。
  1.Senderは少なくとも5x10^4は必要である。
  2.Senderの株によってLuxIの活性が違う。
  2.Senderの株によってLuxIの活性が違う。
 +
-->

Latest revision as of 05:37, 27 October 2007

Chiba logo.png

Home
Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿


Communication Unit

Our Aim

Fig. 13 Quorum sensing for activating receivers!

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig. 14 Constructed circuit of Quorum sensing in our system.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.

Detailed gene circuit is discribed in Fig. 2.

  • more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments: New Parts!!

Recievers

New Parts We Made!!

  • AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006]
    • BBa I729006 circuit.jpg
  • AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005]
    • BBa I729005 circuit.jpg
  • Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004]
    • Chiba BBa I729004.png

From Biobrick

  • BBa_T9002
    • T9002 circuit.jpg

Future Work

  • inverter-aiiA
    • Rec inv aiia.jpg

Senders

New Parts We Made

  • MetK Sender ( could not deposit to registry; sequencing half completed )

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg

Retrieved from "http://2007.igem.org/wiki/index.php/Chiba/Communication"