Calgary/final result

From 2007.igem.org

< Calgary(Difference between revisions)
 
(9 intermediate revisions not shown)
Line 1: Line 1:
<html>
<html>
 +
<!--
 +
  E.co Lisa Final Results Page
 +
  This page summarizes the final product of the E. colLisa project
 +
  It also provides some verification information
 +
-->
<head>
<head>
 +
<!--
 +
  Cascading Style Sheet (CSS) component
 +
  in plain html style sheets are completely independent of the HTML page but are included in this page to simplify design within a wiki script environment.
 +
  This section describes the layout, colors and images displayed on the page
 +
-->
<style>
<style>
Line 65: Line 75:
</head>
</head>
<body>
<body>
 +
<!--
 +
  E.coLisa Title
 +
  The title and and top level navigation links
 +
-->
<table class="header">
<table class="header">
   <tr>
   <tr>
     <td><a href="https://2007.igem.org/Calgary"><img style="border:none" src="https://static.igem.org/mediawiki/2007/3/34/BackToHome.gif" alt="back to U of C Homepage"></a></td>
     <td><a href="https://2007.igem.org/Calgary"><img style="border:none" src="https://static.igem.org/mediawiki/2007/3/34/BackToHome.gif" alt="back to U of C Homepage"></a></td>
     <td><a name="top"> <img src="https://static.igem.org/mediawiki/2007/f/fe/EcoLisaHeader2.gif" /></a></td>
     <td><a name="top"> <img src="https://static.igem.org/mediawiki/2007/f/fe/EcoLisaHeader2.gif" /></a></td>
-
     <td align="right"><a href=""><img img style="border:none" src="https://static.igem.org/mediawiki/2007/2/29/CheckOutevoGEM.gif" alt="Check out evoGEM"></a></td>
+
     <td align="right"><a href="https://2007.igem.org/Calgary/evoGEM_introduction"><img img style="border:none" src="https://static.igem.org/mediawiki/2007/2/29/CheckOutevoGEM.gif" alt="Check out evoGEM"></a></td>
   </tr>
   </tr>
</table>
</table>
 +
<!--
 +
  E.coLisa main menu
 +
  The navigation menu for the E.co Lisa section of the site
 +
-->
<table class="links" >
<table class="links" >
   <tr>
   <tr>
Line 84: Line 102:
   </tr>
   </tr>
</table>
</table>
-
<p>This section summarizes the final results of our work with <em>E. co</em>Lisa.</p>
+
 
 +
<!--
 +
  Final state of project
 +
-->
 +
<p>At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present.  This section summarizes the final results of our work with <em>E. co</em>Lisa.</p>
 +
 
 +
<!--
 +
  Navigation links
 +
  Selecting a link will take the user to the results of the desired selection
 +
-->
 +
<table style="margin-bottom:20px" width="100%">
 +
  <tr>
 +
    <td valign="top"  width="33%"><p><a style="font-size:15px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#contributions" title="See our contributions to the Registry" >Contributions To The Registry</a></td>
 +
    <td valign="top"  width="33%"><p><a style="font-size:15px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#agarase" title="Sequencing of Agarase" >Sequencing of Agarase</a></td>
 +
    <td valign="top"  width="33%"><p> <a style="font-size:15px; font-family:Verdana, Arial, Helvetica, sans-serif;" href="#logic" title="Sequencing of Logic Circut" >Sequencing of Logic Circut </a></td>
 +
  </tr>
 +
</table>
 +
 
 +
<!--
 +
  Table summarizing the parts contributed to the registry
 +
-->
<table width="100%">
<table width="100%">
 +
  <tr>
 +
    <td style="width:85%;"><p style="font-size:20px; font-weight:bold;"><a style="text-decoration:none" name="contributions"> Our Contributions To The Registry</a></p></td>
 +
    <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td>
 +
  </tr>
 +
</table>
 +
<table style="font-size:12px; border:#CCCCCC outset thin" width="100%">
   <tr >
   <tr >
-
     <td>Number</td>
+
     <td><strong>Number</strong></td>
-
     <td> Parts</td>
+
     <td><strong> Parts</strong></td>
-
     <td>Plasmid</td>
+
     <td><strong>Plasmid</strong></td>
-
     <td>New part name</td>
+
     <td><strong>New part name</strong></td>
-
     <td>Type</td>
+
     <td><strong>Type</strong></td>
-
     <td >Notes</td>
+
     <td ><strong>Notes</strong></td>
   </tr>
   </tr>
   <tr>
   <tr>
Line 193: Line 237:
   </tr>
   </tr>
</table>
</table>
-
<p> </p>
+
<p style="margin-bottom:40px">Our team is pleased to report that we have submitted 13 unique parts to the iGEM registry. These parts include the β-agarase gene, our logic circut and several of the intermediate parts we constructed. </p>
 +
 
 +
<!--
 +
  Sequencing information for beta Agarase
 +
-->
 +
<table width="100%">
 +
  <tr>
 +
    <td style="width:85%;"><p style="font-size:20px; font-weight:bold;"><a style="text-decoration:none" name="agarase"> Sequencing of Agarase</a></p></td>
 +
    <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td>
 +
  </tr>
 +
</table>
 +
<p> We were able to sucessfully isolate β-agarase gene from the <em>Pseudoalteromonas atlantica</em> genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format. </p>
 +
<table>
 +
  <tr>
 +
    <td><a href="https://static.igem.org/mediawiki/2007/8/85/Agaraseclone_sequence.pdf" target="_blank"><img style="border:#CCCCCC thin outset" src="https://static.igem.org/mediawiki/2007/9/9f/AgaraseSequencingThumb.gif" atl="sequencing information from β-agarase gene" /></a> </td>
 +
    <td> The sequencing information obtained confirms that we have indeed isolated the β-agarase gene and it is present in the plasmid. </td>
 +
  </tr>
 +
</table>
 +
<table style="margin-bottom:30px">
 +
  <tr>
 +
    <td><img src="https://static.igem.org/mediawiki/2007/b/b6/AgaraseChromatogramForward.jpg" alt="Reverse Chromatogram from aragase" /> </td>
 +
    <td><p> This is the Reverse Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid</p></td>
 +
  </tr>
 +
  <tr>
 +
    <td><img src="https://static.igem.org/mediawiki/2007/7/7a/AgaraseChromatogramForward2%28actuallyForward%29.jpg" atl="Forward Chromatogram from agarase" /> </td>
 +
    <td><p> This is the Forward Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid</p></td>
 +
  </tr>
 +
</table>
 +
 
 +
<!--
 +
  Sequencing informatio for the logic circut
 +
-->
 +
<table width="100%">
 +
  <tr>
 +
    <td style="width:85%;"><p style="font-size:20px; font-weight:bold;"><a style="text-decoration:none" name="logic"> Sequencing of Logic Circut</a></p></td>
 +
    <td width="15%"><a style="float:right;" href="#top" title="return to top">return to top</a> </td>
 +
  </tr>
 +
</table>
 +
<p> There were three seperate logic ciructs prepared. Each differed only in the initial promotorer being used. The first circut used part R0084 a promter that is activated by phosphorylated <em>ompR</em> while the other two R0082 and R0083 are repressed by phosphorylated <em>ompR</em> </p>
 +
<p> Our team synthesized plasmids containing our logic circut using each of these promoters. The links below connect to pdf's showing the result of the sequencing for each of these plasmids. The sequencing information confirmed that our logic circut was present in the plasmids. </p>
 +
<table style="font-size:16px; width:100%">
 +
  <tr>
 +
    <td>|<a href="https://static.igem.org/mediawiki/2007/5/55/R82akgv.pdf" title="R0082 Logic Circut">R0082 Logic Circut</a>| </td>
 +
    <td> |<a href="https://static.igem.org/mediawiki/2007/3/3a/R83AKVF5FR83AKVR.pdf" title="R0083 Logic Circut">R0083 Logic Circut</a>|</td>
 +
    <td>|<a href="https://static.igem.org/mediawiki/2007/a/aa/R84AKGB.pdf" title="R0084 Logic Circut">R0084 Logic Circut</a>|</td>
 +
  </tr>
 +
</table>
</body>
</body>
</html>
</html>

Latest revision as of 06:54, 19 December 2007

back to U of C Homepage Check out evoGEM
Projects Design: Wet Lab Design: Printer Design: Software Testing Construction: The Wetlab Protocols Final Result of E.co Lisa

At the time of the Jamboree the final state of our project is unfortunately unfinished. We were unable to complete our bacterial printer system. However, even without the fully working printer system our team still has many acheivements to present. This section summarizes the final results of our work with E. coLisa.

Contributions To The Registry

Sequencing of Agarase

Sequencing of Logic Circut

Our Contributions To The Registry

 return to top
Number Parts Plasmid New part name Type Notes
1 agarase pSB1A2 i737020 Basic Part
2 R0084 . A340620 . S01414 . I13504 pSB1AK3 I737010 Project
3 R0083 . A340620 . S01414 . I13504 pSB1AK3 I737012 Project
4 R0082 . A340620 . S01414 . I13504 pSB1AK3 I737014 Project
5 R0040 . A340620 . I13504 pSB1AC3 S03868 Intermediate Entered as part I13553 and J26010, not available or experience
6 R0040 . J01010 . I13401 pSB1AC3 S03867 Intermediate Entered as part I714081, but not available
7 R0040 . J01008 . B0015 pSB1AC3 S03863 Intermediate Is S03862 . B0015
8 R0040 . J01080 . I13401 pSB1AC3 S03864 Intermediate Is J01109 . I13401
9 R0040 . J23008 . B0015 pSB1AC3 S03866 Intermediate Is S03865 . B0015
10 R0040 . J01010 . E0040 . S01414 . B0015 pSB1AK3 S03871 Intermediate Is S03870 . I0462
11 S01414 . I13504 pSB1AK3 S03869 Intermediate
12 R0040 . A340620 . J23008 . B0015 pSB1AK3 S03872 Intermediate Is F2620 . J23021
13 R0040 . J06801 pSB2K3 S03743 Intermediate

Our team is pleased to report that we have submitted 13 unique parts to the iGEM registry. These parts include the β-agarase gene, our logic circut and several of the intermediate parts we constructed.

Sequencing of Agarase

 return to top

We were able to sucessfully isolate β-agarase gene from the Pseudoalteromonas atlantica genome. Here are the results we obtained from sequencing the isolated β-agarase gene. Click on the image below to see the full sequencing information for β-agarase in pdf format.

The sequencing information obtained confirms that we have indeed isolated the β-agarase gene and it is present in the plasmid.
Reverse Chromatogram from aragase

This is the Reverse Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid

This is the Forward Chromatogram from the sequencing of our β-agarase containing plasmid. The results confirm that β-agarase is present in the plasmid

Sequencing of Logic Circut

 return to top

There were three seperate logic ciructs prepared. Each differed only in the initial promotorer being used. The first circut used part R0084 a promter that is activated by phosphorylated ompR while the other two R0082 and R0083 are repressed by phosphorylated ompR

Our team synthesized plasmids containing our logic circut using each of these promoters. The links below connect to pdf's showing the result of the sequencing for each of these plasmids. The sequencing information confirmed that our logic circut was present in the plasmids.

|R0082 Logic Circut| |R0083 Logic Circut| |R0084 Logic Circut|

Retrieved from "http://2007.igem.org/wiki/index.php/Calgary/final_result"