BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
_______________________________________________________<br> | _______________________________________________________<br> | ||
Alpha portion<br> | Alpha portion<br> | ||
| - | Pcr CA1150F/CA1099R on I716077 ( | + | Pcr CA1150F/CA1099R on I716077 ()<br> |
Product is ADtemp5<br> | Product is ADtemp5<br> | ||
____________________________________________________________<br> | ____________________________________________________________<br> | ||
Revision as of 01:19, 2 July 2007
Beta portion
pcr CA1154F/G00101 on 9145-I716072 (575 bp)
Product is ADtemp1
Sub ADtemp1 into pTRC plasmid with (Bgl2/XhoI)
Product is ADtemp2
pcr CA1099F/D58 on ADtemp2 (3465 bp)
Product is ADtemp3
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Alpha portion
Pcr CA1150F/CA1099R on I716077 ()
Product is ADtemp5
____________________________________________________________
Digest ADtemp3 (BsaI/BamHI)
Digest ADtemp5 (BsaI/BglII)
Ligate, product is: I716074 (Best yield)
