OtsA-construction file
From 2007.igem.org
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| - | PCR otsAF/otsAIR on otsA (413 bp, gp=A) | + | PCR otsAF/otsAIR on otsA (413 bp, gp=A)<br> |
| - | PCR otsAIF/otsAR on otsA (1077 bp, gp=B) | + | PCR otsAIF/otsAR on otsA (1077 bp, gp=B)<br> |
-------------------------------------------- | -------------------------------------------- | ||
| - | PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI) | + | PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI)<br> |
| - | Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L) | + | Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)<br> |
| - | Product pBca9145-BBa I716301 | + | Product pBca9145-BBa I716301<br> |
| - | -------------------------------------------- | + | --------------------------------------------<br> |
| - | otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC | + | otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC<br> |
| - | otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG | + | otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG<br> |
| - | otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG | + | otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG<br> |
| - | otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC | + | otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC<br> |
Latest revision as of 17:57, 2 July 2007
PCR otsAF/otsAIR on otsA (413 bp, gp=A)
PCR otsAIF/otsAR on otsA (1077 bp, gp=B)
PCR otsAF/otsAR on A+B (1456 bp BglII/XhoI)
Digest pBca9145-Bca1144 (BglII/XhoI 2054+910, L)
Product pBca9145-BBa I716301
otsAF forward BglII site anneals to otsA cgtaaAGATCTATGAGTCGTTTAGTCGTAGTATC
otsAIF Removing BamHI site in otsA CGATGACATTATCTGGATtCACGATTATCACCTG
otsAIR Removing BamHI site in otsA CAGGTGATAATCGTGaATCCAGATAATGTCATCG
otsaR reverse XhoI site anneals to otsA cgtatCTCGAGttaGGATCCTTACGCAAGCTTTGGAAAGGTAGC
