BerkiGEM2007-AustinConstructionFile21
From 2007.igem.org
(Difference between revisions)
Line 5: | Line 5: | ||
Beta: <br> | Beta: <br> | ||
- | PCR | + | PCR CA1099R/D58 on Part Ptrc-I716080 <br> |
Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | ||
<br><br> | <br><br> | ||
Product is pTRC-I716071 | Product is pTRC-I716071 |
Revision as of 21:38, 2 July 2007
Alpha:
PCR CA1150/CA1099R on I716070 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.
Beta:
PCR CA1099R/D58 on Part Ptrc-I716080
Digest (Bsa1/BamH1) and ligate with alpha portion.
Product is pTRC-I716071