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Revision as of 22:05, 3 July 2007 (view source) PhillipDodson (Talk | contribs) m (Melb:Primary Reagents & disposables moved to Melbourne/Primary Reagents & disposables) ← Older edit		Revision as of 04:51, 4 July 2007 (view source) PhillipDodson (Talk | contribs) Newer edit →
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	===Enzymes===		===Enzymes===
-	*Restriction enzymes (in particular, NotI, EcoRI, XbaI, HindIII)	+	*[[Melbourne/primary Restriction enzymes]]
-	*Ligase	+	** NotI,
-	*Competent cells (both for plasmid purification and protein expression)	+	**EcoRI,
-	*DNA standard	+	**XbaI,
		+	**HindIII,
		+	**SpeI,
		+	*[[Melbourne/primary Ligase|Ligase]]
		+	*[[Melbourne/primary competant DH5a|DH5a Competent cells]]
		+	*[[Melbourne/primary DNA marker|DNA ladder]]
	===Chemicals===		===Chemicals===
-	*Ingredients for media (LB, SOB)	+	*[[Melbourne/primary agar|Agar]]
-	*Glycerol	+	*[[Melbourne/primary Agarose|Agarose]]
-	*Buffers (including TAE and TE)	+	*[[Melbourne/primary EB|Ethidium Bromide]]
-	*Loading dye for DNA gel	+	*[[Melbourne/primary EDTA|EDTA]]*[[Melbourne/primary glycerol|Glycerol]]
		+	*[[Melbourne/primary dna loading|Loading dye for DNA gel]]
		+	*[[Melbourne/primary Nacl|NaCl]]
		+	*[[Melbourne/primary Tris|TRIS]]
		+	===Other===
		+	*[[Melbourne/primary ice|ice]]
		+	*[[Melbourne/primary milliq|milliQ water]]
		+	*[[Melbourne/primary distilled|Distilled water]]
	==Disposables==		==Disposables==
		+	*[[Melbourne/primary Parrafilm|Parrafilm]]
	===TUBES:===		===TUBES:===
-	*Falcon: 10ml, 50ml	+	*[[Melbourne/primary falcon|Falcon: 10ml, 50ml]]
	*microcentrifuge: 1.7ml		*microcentrifuge: 1.7ml
	*PCR		*PCR

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Revision as of 04:51, 4 July 2007

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Contents 1 Primary Reagents 1.1 Kits 1.2 Enzymes 1.3 Chemicals 1.4 Other 2 Disposables 2.1 TUBES: 2.2 Tips 2.3 Other

Primary Reagents

Kits

• Miniprep kit
• Gel clean-up system
• Site directed mutagenesis (stratagene)
  • Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
  • Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
  • QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
• Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx

Enzymes

• Melbourne/primary Restriction enzymes
  • NotI,
  • EcoRI,
  • XbaI,
  • HindIII,
  • SpeI,
• Ligase
• DH5a Competent cells
• DNA ladder

Chemicals

• Agar
• Agarose
• Ethidium Bromide
• EDTA*Glycerol
• Loading dye for DNA gel
• NaCl
• TRIS

Other

• ice
• milliQ water
• Distilled water

Disposables

• Parrafilm

TUBES:

• Falcon: 10ml, 50ml
• microcentrifuge: 1.7ml
• PCR

Tips

• Tips (10, 200, 1000 ul)

Other

• Gloves (Large , Med, Small)
• Scalpels
• Tube racks
• Freezer boxes
• Parafilm