Melbourne/Primary Reagents & disposables
From 2007.igem.org
(Difference between revisions)
| Line 25: | Line 25: | ||
*[[Melbourne/primary Agarose|Agarose]] | *[[Melbourne/primary Agarose|Agarose]] | ||
*[[Melbourne/primary EB|Ethidium Bromide]] | *[[Melbourne/primary EB|Ethidium Bromide]] | ||
| - | *[[Melbourne/primary EDTA|EDTA]]*[[Melbourne/primary glycerol|Glycerol]] | + | *[[Melbourne/primary EDTA|EDTA]] |
| + | *[[Melbourne/primary glycerol|Glycerol]] | ||
*[[Melbourne/primary dna loading|Loading dye for DNA gel]] | *[[Melbourne/primary dna loading|Loading dye for DNA gel]] | ||
*[[Melbourne/primary Nacl|NaCl]] | *[[Melbourne/primary Nacl|NaCl]] | ||
Revision as of 05:53, 4 July 2007
<Back to team home page> <Template>
Contents |
Primary Reagents
Kits
- Miniprep kit
- Gel clean-up system
- Site directed mutagenesis (stratagene)
- Quickchange multi 200515(x10),200514(x30): 3-5 changes 1 primer each 50% <8Kb
- Quickchange 200518(x30) 200519(x10): single change 2 primers 90% <8Kb
- QuickchangeII XL 200522(x30) 200521(x10): single change 2 primers 80% >8Kb
- Primer design program : http://www.stratagene.com/sdmdesigner/default.aspx
Enzymes
- Melbourne/primary Restriction enzymes
- NotI,
- EcoRI,
- XbaI,
- HindIII,
- SpeI,
- Ligase
- DH5a Competent cells
- DNA ladder
Chemicals
Other
Disposables
- Parrafilm
- Tips (10, 200, 1000 ul)
- Gloves (Large , Med, Small)
- Scalpels
- Tube racks
- Freezer boxes
TUBES:
- Falcon: 10ml, 50ml
- microcentrifuge: 1.7ml
- PCR
