BerkiGEM2007-AustinConstructionFile24
From 2007.igem.org
(Difference between revisions)
| Line 1: | Line 1: | ||
Alpha: <br> | Alpha: <br> | ||
| - | PCR CA1150/CA1099R on I716077 (1727 Bp)<br> | + | PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)<br> |
Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br> | Digest (Bsa1/Bgl2) and ligate with beta portion.<br> <br> | ||
Beta: <br> | Beta: <br> | ||
| - | PCR | + | PCR CA1099F/G00101 on Part 9145-I716081 <br> |
Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | Digest (Bsa1/BamH1) and ligate with alpha portion. <br> | ||
<br><br> | <br><br> | ||
| - | Product is | + | Product is 9145-I716074 (best yield) |
Revision as of 00:53, 5 July 2007
Alpha:
PCR CA1150/CA1099R on 9145-I716077 (1727 Bp)
Digest (Bsa1/Bgl2) and ligate with beta portion.
Beta:
PCR CA1099F/G00101 on Part 9145-I716081
Digest (Bsa1/BamH1) and ligate with alpha portion.
Product is 9145-I716074 (best yield)
