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Revision as of 06:45, 11 July 2007 (view source) PhillipDodson (Talk | contribs) ← Older edit		Revision as of 06:52, 11 July 2007 (view source) PhillipDodson (Talk | contribs) Newer edit →
Line 8:		Line 8:
	!Enzyme		!Enzyme
	!Cut site		!Cut site
		+	!100% buffers
	!Cost		!Cost
	!No of U		!No of U
Line 16:		Line 17:
	!PstI		!PstI
	|5CTGCA^G3		|5CTGCA^G3
		+	|
	|US$58		|US$58
	|10,000 @ 20,000U/ml		|10,000 @ 20,000U/ml
Line 24:		Line 26:
	!EcoRI		!EcoRI
	|5G^AATTC3		|5G^AATTC3
		+	|
	|US$53		|US$53
	|10,000 @ 20,000U/ml		|10,000 @ 20,000U/ml
Line 32:		Line 35:
	!HaeII		!HaeII
	|5RGCGC^Y3		|5RGCGC^Y3
		+	|
	|US$53		|US$53
	|2,000U @ 20,000U/ml		|2,000U @ 20,000U/ml
Line 40:		Line 44:
	!SalI		!SalI
	|5G^TCGAC3		|5G^TCGAC3
		+	|
	|US$56		|US$56
	|2,000U @ 20,000U/ml		|2,000U @ 20,000U/ml
Line 48:		Line 53:
	!XbaI		!XbaI
	|5T^CTAGA3		|5T^CTAGA3
		+	|
	|US$63		|US$63
	|3,000U @ 20,000U/ml		|3,000U @ 20,000U/ml
Line 54:		Line 60:
	|YES		|YES
	|}		|}
		+
		+	====Double Digest Buffers====
		+
		+
		+
	====Lab Location====		====Lab Location====
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	====Store====		====Store====
-	*Available from Bio21 store? Yes	+	*Available from Bio21 store? Yes
		+	*location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see [[Media:melbourne daisy |Daisy]]).
-	====Supplier:====
-	*Supplier:
-	*Suppliers part no:
-	*Contact details for supplier:
-	*Email:
-	*Phone:
	====Manufacturer:====		====Manufacturer:====

---

Revision as of 06:52, 11 July 2007

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Contents 1 Physical data 2 Double Digest Buffers 3 Lab Location 4 Store 5 Manufacturer:

Physical data

• Description: NEB Restriction enzymes are used generally in the Lab.

NEB restriction enzymes

Enzyme	Cut site	100% buffers	Cost	No of U	Part no/data	location	In store
PstI	5CTGCA^G3		US$58	10,000 @ 20,000U/ml	[http://www.neb.com/nebecomm/products/productR0140.asp NEB.R0140S]		YES
EcoRI	5G^AATTC3		US$53	10,000 @ 20,000U/ml	[http://www.neb.com/nebecomm/products/productR0101.asp NEB.R0101S]		YES
HaeII	5RGCGC^Y3		US$53	2,000U @ 20,000U/ml	[http://www.neb.com/nebecomm/products/productR0107.asp NEB.R0107S]		YES
SalI	5G^TCGAC3		US$56	2,000U @ 20,000U/ml	[http://www.neb.com/nebecomm/products/productR0138.asp NEB.R0138S]		YES
XbaI	5T^CTAGA3		US$63	3,000U @ 20,000U/ml	[http://www.neb.com/nebecomm/products/productR0145.asp NEB.R0145S]		YES

Double Digest Buffers

Lab Location

• Location see table
• Permision to use required? No

Store

• Available from Bio21 store? Yes
• location: [Media:melbourne Erefrigerator |E-refrigerator] Needs account card. (see Daisy).

Manufacturer:

• Manufacturer:NEB
• complete listing of available enzymes

• PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007