Template:BerkiGEM2007 ArthurSequencingFiles

From 2007.igem.org

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|AY031||071107||I716012 (101-008)||2||ca998||???||[[BerkiGEM2007-Sequencing-AY031 | AY031]]
|AY031||071107||I716012 (101-008)||2||ca998||???||[[BerkiGEM2007-Sequencing-AY031 | AY031]]
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|AY032||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY032 | AY032]]
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|AY032||071207||I716013 (pBca9145-yfbE-rbs-ATG)||1||ca998||???||[[BerkiGEM2007-Sequencing-AY032 | AY032]]
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|AY033||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY033 | AY033]]
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|AY033||071207||I716013 (pBca9145-yfbE-rbs-ATG)||3||ca998||???||[[BerkiGEM2007-Sequencing-AY033 | AY033]]
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|AY034||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY034 | AY034]]
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|AY034||071207||I716014 (pBca9145-yfbE_solo)||1||ca998||???||[[BerkiGEM2007-Sequencing-AY034 | AY034]]
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|AY035||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY035 | AY035]]
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|AY035||071207||I716014 (pBca9145-yfbE_solo)||2||ca998||???||[[BerkiGEM2007-Sequencing-AY035 | AY035]]
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|AY036||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY036 | AY036]]
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|AY036||071207||I716014 (pBca9145-yfbE_solo)||3||ca998||???||[[BerkiGEM2007-Sequencing-AY036 | AY036]]
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|AY037||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY037 | AY037]]
|AY037||???||???||???||???||???||[[BerkiGEM2007-Sequencing-AY037 | AY037]]

Revision as of 22:56, 12 July 2007

SampleDatePlasmidClonePrimerResultFile Link
AY001060407 pBca9145-Bca1145#1ca998Probably good. Long, ambiguous long AAAAAAA. AY001
AY002060407 pBca9145-Bca1145#2ca998Good. AY002
AY003061207pBca9145-HPI/katG (I716253)A1ca998super wrong. Seems like only half of HPI/katG, from the AY004.Sal.katG.HPI-Fx "mutate GAT>GAC (BglII)" til the end, was cloned. AY003
AY004061207pBca9145-HPI/katG (I716253)A3ca998generally OK. There is one extra G at 584 in the goal plasmid in the calls file. There is low confidence, but I am not sure what to make of it. Possibly should send for recheck at this point; may be necessary to check the mutation point @1308, and the end portion as well. But based on the miniprep digest run yesterday, this guy has a high chance of being the right one. DECISION: sent as AY007 with G01001 for a reverse check. AY004
AY005061207pBca9145-HPI/katG (I716253)A4ca998crazy :( there are big gaps AY005
AY006061207pBca9145-wbbL (I716000)B1ca998also crazy AY006
AY007061307pBca9145-HPI/katG (I716???)A3G01001Looks good actually, only notable thing is silent point mutation @ 1782 TCG > TCA still S. but the mutation point was just missed by sequencing. I don't think it's necessary to recheck; i have faith the primers worked right. well.. idk. major concern still is the possible addition mutation of a G AY007
AY008061307pBca9145-neuS (I716001)C1ca998Forward. Looks great! AY008
AY009061307pBca9145-neuS (I716001)C1G01001Reverse. Looks great! AY009
AY010061407I716002 (pBca9145-yfbE_promote)D1ca998Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C AY010
AY011061407I716002 (pBca9145-yfbE_promote)D1G01001Mixed DNA tube (contamination) AY011
AY012061407I716002 (pBca9145-yfbE_promote)D4ca998Perfect promoter cloned region. @ 443 there is a deletion of ttgcag. Point mutation @631, T > C. @640 addition of a C AY012
AY013061407I716002 (pBca9145-yfbE_promote)D4G01001Mixed DNA tube (contamination) AY013
AY014061507I716000 (pBca9145-wbbL)E1ca998long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T @ 812, but read quality is low here. Lack of agttgca right after insert?? maybe not?? AY014
AY015061507I716000 (pBca9145-wbbL)E2ca998long stretch of t's at 696, sequencing got an extra one. Likely read error. Missing a T at 801. Lack of agttgca right after insert?? maybe not?? AY015
AY016061807I716000 (pBca9145-wbbL)E1G01001good AY016
AY017061807I716000 (pBca9145-wbbL)E2G01001good AY017
AY018061807I716253 (pBca9145-HPI/katG)A3ay007good AY018
AY019062107I716006 (pBca9145-Bca9203 B5red)G1ca998fail, mixed sample?? I believe it's quintara's fault AY019
AY020062107I716006 (pBca9145-Bca9203 B5red)G3ca998fail, mixed sample?? I believe it's quintara's fault AY020
AY021062507I716006 (pBca9145-Bca9203 B5red)G1ca998looks good AY021
AY022062507I716006 (pBca9145-Bca9203 B5red)H3ca998looks good AY022
AY023062507I716005 (pBca9145-Bca9229 cytoB5)H3ca998looks good AY023
AY024062507I716005 (pBca9145-Bca9229 cytoB5)H4ca998looks good AY024
AY025062807I716002 (pBca9145-yfbE_promote)IBca998our proprietary yfbE was cloned successfully AY025

Start 26

AY026062807I716002 (pBca9145-yfbE_promote)IDca998our proprietary yfbE was cloned successfully AY026
AY027070507I716008 (Ptet-rbs-wbbL-rbs-neuS)J1ca998wbbL or neuS not cloned. only Ptet. AY027
AY028070507I716008 (Ptet-rbs-wbbL-rbs-neuS)J2ca998wbbL or neuS not cloned. only Ptet. AY028
AY029070507I716008 (Ptet-rbs-wbbL-rbs-neuS)J3ca998Ptet and wbbL were successfully cloned. neuS, unsure. Possibly neuS is gone since there could be a BamHI site right after wbbL in sequence file. AY029
AY030071107I716012 (101-008)1ca998??? AY030
AY031071107I716012 (101-008)2ca998??? AY031
AY032071207I716013 (pBca9145-yfbE-rbs-ATG)1ca998??? AY032
AY033071207I716013 (pBca9145-yfbE-rbs-ATG)3ca998??? AY033
AY034071207I716014 (pBca9145-yfbE_solo)1ca998??? AY034
AY035071207I716014 (pBca9145-yfbE_solo)2ca998??? AY035
AY036071207I716014 (pBca9145-yfbE_solo)3ca998??? AY036
AY037??????????????? AY037
AY038??????????????? AY038
AY039??????????????? AY039
AY040??????????????? AY040
AY041??????????????? AY041
AY042??????????????? AY042
AY043??????????????? AY043
AY044??????????????? AY044
AY045??????????????? AY045
AY046??????????????? AY046
AY047??????????????? AY047
AY048??????????????? AY048
AY049??????????????? AY049
AY050??????????????? AY050
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