Paris/Oligos

From 2007.igem.org

(Difference between revisions)
Line 44: Line 44:
DPst1-DGAT-R          AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
DPst1-DGAT-R          AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
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{|
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|- style="background: #ccccff;"
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! colspan="5" style="background: #ccccff;" | Table of Oligos
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|- style="background: #ccccff; text-align: center;"
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|width=5%| Number
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|width=5%| Name
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|width=60%| Sequence
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|width=5%| Length
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|width=25%| Comments
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|- style="background: #cccccc;" 
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| style="background: #ccffcc;" | 1
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| Lox71-FtsZ-F
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| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
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| 89
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 2
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| FtsZ-R
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| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT
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| 49
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 3
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| Lox71-FtsA-F
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| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
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| 89
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 4
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| DEcoR1-FtsZ-R
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| CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC
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| 40
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 5
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| DEcoR1-FtsZ-F
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| TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA
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| 40
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 6
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| Lox66-DapAColi-F
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| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT
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| 84
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 7
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| DapAColi-R           
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| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC
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| 52
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 8
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| Lox66-DapASubtilis-F 
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| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA
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| 84
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 9
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| DapASubtilis-R       
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| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
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| 52
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 10
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| Lox71-KmR-F         
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| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
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| 85
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|
 +
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 11
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|
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|
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|
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|
 +
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 12
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|
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|
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|
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|
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|- style="background: #cccccc;"
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| style="background: #ccffcc;" | 13
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|
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|
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|
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|
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|}

Revision as of 12:53, 13 July 2007

Constructions

  • Lox71-FtsZ:

Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89

FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49

  • Lox71-FtsA-FtsZ:

Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89

  • Deletion of Ecor1 site in FtsZ:

DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40

DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40


  • Lox66-DapAColi:

Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84

DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52

  • Lox66-DapASubtilis:

Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84

DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52


  • Lox71-KmR-Lox66:

Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85

Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86


  • Deletion of Pst1 site in DGAT:

DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40

DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40

Table of Oligos
Number Name Sequence Length Comments
1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84
9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
11
12
13
Retrieved from "http://2007.igem.org/wiki/index.php/Paris/Oligos"