Melbourne/GvpL site dirrected primers
From 2007.igem.org
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Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html | Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html | ||
{|border="1" | {|border="1" | ||
- | !Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches !!GC content !!Tm ver!!OD=1 | + | !Primer Name !!Length (nt.) !!Tm !!Duplex Energy at 68°C !!Energy Cost of Mismatches !!GC content !!Tm ver!!OD=1!!bp before!!bp after |
|- | |- | ||
- | |g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% || 41% || | + | |g213a ||39 ||78.45°C ||-44.89 kcal/mole ||6.5% || 41% || 81 ||2216nM||19||19 |
|- | |- | ||
- | |g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole||7.8% || 41% || | + | |g213a_antisense ||39 ||78.45°C ||-42.10 kcal/mole||7.8% || 41% || 81 ||2216nM|| || |
|- | |- | ||
- | |g318a ||47 ||78.97°C ||-46.02 kcal/mole || 6.3%|| | + | |g318a ||47 ||78.97°C ||-46.02 kcal/mole || 6.3%|| 30%|| 80 || 1754nM||25||21 |
|- | |- | ||
- | |g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7% || | + | |g318a_antisense ||47 ||78.97°C ||-42.40 kcal/mole ||7.7% || 30% || 80 || 1754nM|| || |
|- | |- | ||
- | |g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% || | + | |g351a ||46 ||78.02°C ||-43.40 kcal/mole ||5.3% || 33% || 80 ||1820nM||26||19 |
|- | |- | ||
- | |g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4%|| 33% || | + | |g351a_antisense ||46 ||78.02°C ||-40.27 kcal/mole ||7.4%|| 33% || 80 ||1820nM|| || |
|- | |- | ||
- | |g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4%|| 41% || | + | |g696a ||39 ||78.45°C ||-42.46 kcal/mole ||5.4%|| 41% ||81||2496nM||19||19 |
|- | |- | ||
- | |g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% || 41% || | + | |g696a_antisense ||39 ||78.45°C ||-43.97 kcal/mole ||6.8% || 41% ||81 ||2496nM|| || |
|} | |} |
Latest revision as of 02:27, 18 July 2007
Primer sequences: Primer Name Primer Sequence (5' to 3')
g213a | 5'-aggatatgagctggctgcaagaaaaagcatttcatcatc-3' |
g213a_antisense | 5'-gatgatgaaatgctttttcttgcagccagctcatatcct-3' |
g318a | 5'-ttataaaggtgaagaaagtctgcaagcagctattgagattaacaaag-3' |
g318a_antisense | 5'-ctttgttaatctcaatagctgcttgcagactttcttcacctttataa-3' |
g351a | 5'-attgagattaacaaagaaaagatagaaaattcactgacgctgcttc-3' |
g351a_antisense | 5'-gaagcagcgtcagtgaattttctatcttttctttgttaatctcaat-3' |
g696a | 5'-ttcttctcccgtctctgcaaattactaagttcgtaaacg-3' |
g696a_antisense | 5'-cgtttacgaacttagtaatttgcagagacgggagaagaa-3' |
Oligonucleotide information: Tm and CG content verified from http://mbcf.dfci.harvard.edu/docs/oligocalc.html
Primer Name | Length (nt.) | Tm | Duplex Energy at 68°C | Energy Cost of Mismatches | GC content | Tm ver | OD=1 | bp before | bp after |
---|---|---|---|---|---|---|---|---|---|
g213a | 39 | 78.45°C | -44.89 kcal/mole | 6.5% | 41% | 81 | 2216nM | 19 | 19 |
g213a_antisense | 39 | 78.45°C | -42.10 kcal/mole | 7.8% | 41% | 81 | 2216nM | ||
g318a | 47 | 78.97°C | -46.02 kcal/mole | 6.3% | 30% | 80 | 1754nM | 25 | 21 |
g318a_antisense | 47 | 78.97°C | -42.40 kcal/mole | 7.7% | 30% | 80 | 1754nM | ||
g351a | 46 | 78.02°C | -43.40 kcal/mole | 5.3% | 33% | 80 | 1820nM | 26 | 19 |
g351a_antisense | 46 | 78.02°C | -40.27 kcal/mole | 7.4% | 33% | 80 | 1820nM | ||
g696a | 39 | 78.45°C | -42.46 kcal/mole | 5.4% | 41% | 81 | 2496nM | 19 | 19 |
g696a_antisense | 39 | 78.45°C | -43.97 kcal/mole | 6.8% | 41% | 81 | 2496nM |