Paris/Modeling char
From 2007.igem.org
< Paris(Difference between revisions)
| (3 intermediate revisions not shown) | |||
| Line 3: | Line 3: | ||
* The promoters: | * The promoters: | ||
| - | + | -DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of | |
| - | + | repression. look the arabinose AraC | |
| + | |||
| + | -the high constitutive promoter that we are using:<bbpart>BBa_J23112</bbpart> | ||
*cre efficience : | *cre efficience : | ||
| + | |||
| + | |||
| + | <bbpart>BBa_J5528</bbpart> :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct | ||
| + | |||
| + | assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency | ||
| + | |||
| + | |||
| + | [[Paris/Modeling|<<Back to modelling]] | ||
Latest revision as of 15:18, 19 July 2007
We must characterise the different part we are using. Essentially :
- The promoters:
-DAPap: we must study the basic pops when no DAP is present, and establish the pops output curve depending of repression. look the arabinose AraC
-the high constitutive promoter that we are using:BBa_J23112
- cre efficience :
BBa_J5528 :Arabinose -> pBad = GFP : use it to test pbad efficiency,or make a similar construct
assemble Arabinose and cre one one plasmide, loxP ccdb loxP on another, and test the recombination frequency
